Evidence map›Paper›PMID 39354307›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

A Method for Analysis of Oxidized Phospholipids from Biological Samples Using Mass Spectrometry.

Mohan Ghorasaini, Daniela Costa, Victoria J Tyrrell, Majd Protty, Martin Giera, Valerie B O'Donnell

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Mohan GhorasainiCenter for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands.
Daniela CostaDivision of Infection and Immunity, School of Medicine, Cardiff University, Cardiff, UK.
Victoria J TyrrellDivision of Infection and Immunity, School of Medicine, Cardiff University, Cardiff, UK.
Majd ProttyDivision of Infection and Immunity, School of Medicine, Cardiff University, Cardiff, UK.
Martin GieraCenter for Proteomics and Metabolomics, Leiden University Medical Center, Leiden, The Netherlands.
Valerie B O'DonnellDivision of Infection and Immunity, School of Medicine, Cardiff University, Cardiff, UK. o-donnellvb@cardiff.ac.uk.

Funding

British Heart Foundation RG/F/20/110020Wellcome Trust 216278/Z/19/Z
6 · The paper itself

Abstract

Oxidized phospholipids (oxPLs) are generated during innate immunity and inflammation, where they play a variety of biological roles, including regulation of autoimmunity and coagulation. Some are generated by enzymatic reactions, leading to stereo- and regiospecificity, while many others can be formed through nonenzymatic oxidation and truncation and can be used as biomarkers of oxidative stress. Mass spectrometry methods have been developed over many years for oxPL analysis, which can provide robust estimations of molecular species and amounts, where standards are available. Here we present a method used for the analysis of enzymatically-generated oxPL (eoxPL), which allows quantification of mono-hydroxy oxylipin-containing species. We also show profiling of many other partially characterized structures in tissue samples and provide typical chromatograms obtained.

Indexed as

Mass SpectrometryOxidation-ReductionPhospholipidsAnimalsBiomarkersHumansOxidative StressOxylipinsBiomarkersOxylipinsPhospholipidsInflammatory mediatorsLiquid chromatographyMass spectrometryOxidized phospholipidsOxylipins

Identifiers

PMID39354307

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.