Evidence map›Paper›PMID 39354304›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Quantification of Total Ketone Bodies in Biological Matrices Using Stable Isotope-Labeled Internal Standards and RP-UHPLC-MS/MS.

Eric D Queathem, Alisa B Nelson, Patrycja Puchalska

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Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Eric D QueathemDivision of Molecular Medicine, Department of Medicine, University of Minnesota, Minneapolis, MN, USA.
Alisa B NelsonDivision of Molecular Medicine, Department of Medicine, University of Minnesota, Minneapolis, MN, USA.
Patrycja PuchalskaDivision of Molecular Medicine, Department of Medicine, University of Minnesota, Minneapolis, MN, USA. ppuchals@umn.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Acetoacetate (AcAc) and D-beta-hydroxybutyrate (D-βOHB), the two major ketone bodies found in circulation, are linked to multiple physiological and pathophysiological states. Therefore, analytical methodologies surrounding the quantification of total ketone body (TKB) concentrations in biological matrices are paramount. Traditional methods to quantify TKBs relied on indirect spectrophotometric assays with narrow dynamic ranges, which have been significantly improved upon by modern mass spectrometry (MS)-based approaches. However, the lack of stable isotope-labeled internal standards (ISs) for AcAc and the need to distinguish D-βOHB from its closely related structural and enantiomeric isomers pose significant obstacles. Here, we provide a protocol to synthesize and quantify a [

Indexed as

Carbon IsotopesIsotope LabelingTandem Mass Spectrometry3-Hydroxybutyric AcidAcetoacetatesAnimalsChromatography, High Pressure LiquidChromatography, Reverse-PhaseHumansKetone BodiesReference Standards3-Hydroxybutyric AcidAcetoacetatesacetoacetic acidCarbon-13Carbon IsotopesKetone BodiesAcetoacetateD-β-hydroxybutyrateKetone bodiesL-β-hydroxybutyrateStable isotope-labeled internal standardsTandem mass spectrometryUltra-high performance liquid chromatography

Identifiers

PMID39354304

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.