Evidence map›Paper›PMID 39354113›Full record

ArticleApplied microbiology and biotechnology2024

Random mutagenesis and disulfide bond formation improved thermostability in microbial transglutaminase.

Mototaka Suzuki, Masayo Date, Tatsuki Kashiwagi, Kazutoshi Takahashi, Akira Nakamura, Masaru Tanokura, Eiichiro Suzuki, Keiichi Yokoyama

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Applied microbiology and biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Mototaka SuzukiInstitute for Innovation, Ajinomoto Co., Inc., 1-1, Suzuki-Cho, Kawasaki-Shi, Kanagawa, 210-8681, Japan.
Masayo DateInstitute for Innovation, Ajinomoto Co., Inc., 1-1, Suzuki-Cho, Kawasaki-Shi, Kanagawa, 210-8681, Japan.
Tatsuki KashiwagiInstitute for Innovation, Ajinomoto Co., Inc., 1-1, Suzuki-Cho, Kawasaki-Shi, Kanagawa, 210-8681, Japan.
Kazutoshi TakahashiInstitute for Innovation, Ajinomoto Co., Inc., 1-1, Suzuki-Cho, Kawasaki-Shi, Kanagawa, 210-8681, Japan.ORCID http://orcid.org/0000-0003-3275-4109
Akira NakamuraDepartment of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo, 113-8657, Japan.
Masaru TanokuraDepartment of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-Ku, Tokyo, 113-8657, Japan.
Eiichiro SuzukiInstitute for Innovation, Ajinomoto Co., Inc., 1-1, Suzuki-Cho, Kawasaki-Shi, Kanagawa, 210-8681, Japan.
Keiichi YokoyamaInstitute for Innovation, Ajinomoto Co., Inc., 1-1, Suzuki-Cho, Kawasaki-Shi, Kanagawa, 210-8681, Japan. keiichia.yokoyama.d7e@asv.ajinomoto.com.ORCID http://orcid.org/0000-0002-6636-4478

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Microbial transglutaminase (MTG) from Streptomyces mobaraensis is widely used in the food and pharmaceutical industries for cross-linking and post-translational modification of proteins. It is believed that its industrial applications could be further broadened by improving its thermostability. In our previous study, we showed that the introduction of structure-based disulfide bonds improved the thermostability of MTG, and we succeeded in obtaining a thermostable mutant, D3C/G283C, with a T

Indexed as

DisulfidesEnzyme StabilityStreptomycesTransglutaminasesAmino Acid SubstitutionBacterial ProteinsHot TemperatureKineticsMutagenesisTemperatureBacterial ProteinsDisulfidesTransglutaminasesDisulfide bridgeHeat resistanceMicrobial transglutaminaseRandom mutationX-ray crystallography

Identifiers

PMID39354113
PMCPMC11445358

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.