ArticleNPJ science of food2024
Evaluation of enzymatic protocols to optimize efficiency of bovine adipose tissue-derived mesenchymal stromal cell isolation.
Article in NPJ science of food, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Protocol to maximize single-nuclei multiome library complexity for limited primary tissues via optimized sample preparation and library synthesis.STAR protocols · 2026Article
- Assessing the ability of bovine mesenchymal stem cells to grow on okara-based scaffolds derived from soy milk waste.Frontiers in nutrition · 2026Article
- Development of a sustainable strategy for cultured fat production based on serum-free 3D culture of bovine adipose stem cells.Scientific reports · 2025Article
- Establishing protocols for the efficient expansion of canine and feline adipose tissue-derived mesenchymal stromal cells following cell isolation.BMC veterinary research · 2025Article
- Pooled CRISPR screens identifies key regulators of bovine stem cell expansion for cultured meat.Communications biology · 2025Article
- Donor age and breed determine mesenchymal stromal cell characteristics.Stem cell research & therapy · 2025Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sustainable food provision for a continuously growing human population is one of the major challenges for the next decades. Cultured meat represents one of the alternatives which is currently extensively explored. Yet, the most appropriate cell type, capable of long-term proliferation and myogenic differentiation, remains to be identified. Bovine mesenchymal stromal cells (MSCs) are considered as a promising cell source. Within the context of cultured meat production, it is mandatory to maximize cell yield per tissue source. Although many enzymatic methods to isolate MSCs from adipose tissue (AT) have been described, cell yield has never been compared. In this study, we evaluate 32 isolation conditions including four enzyme mixtures (Collagenase type I, Collagenase type I + Trypsin, Liberase
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Registered trials
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