Evidence map›Paper›PMID 39352683›Full record

ArticlePhotochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology2024

A novel fluorescent probe for viscosity and polarity detection in real tobacco root cells and biological imaging.

Yuan He, Long-Ke Li, Mao-Hua Wang, Jing-Ruo Tian, Guo-Yu Chen, Jian-Yong Wang

Abstract read
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In one paragraph

Article in Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Yuan HeCollege of Tobacco Science and Engineering, Zhengzhou University of Light Industry, Zhengzhou, 450002, People's Republic of China.
Long-Ke LiCollege of Tobacco Science and Engineering, Zhengzhou University of Light Industry, Zhengzhou, 450002, People's Republic of China.
Mao-Hua WangFaculty of Light Industry, State Key Laboratory of Biobased Material and Green Papermaking, Key Laboratory of Paper Science and Technology of Ministry of Education, Qi Lu University of Technology (Shandong Academy of Sciences), Jinan, 250353, People's Republic of China.
Jing-Ruo TianDepartment of Cuisine and Nutrition, Shandong College of Tourism and Hospitality, Jinan, People's Republic of China.
Guo-Yu ChenCollege of Tobacco Science and Engineering, Zhengzhou University of Light Industry, Zhengzhou, 450002, People's Republic of China.
Jian-Yong WangFaculty of Light Industry, State Key Laboratory of Biobased Material and Green Papermaking, Key Laboratory of Paper Science and Technology of Ministry of Education, Qi Lu University of Technology (Shandong Academy of Sciences), Jinan, 250353, People's Republic of China. wjy@qlu.edu.cn.ORCID http://orcid.org/0000-0002-5254-1525

Funding

NSFC 21801145
6 · The paper itself

Abstract

The disruption of lipid droplet function is associated with the pathogenesis of various diseases. Clarifying the response behavior of lipid droplets to the microenvironment at the cellular level is of great significance. Plant lipids not only exist in phospholipids in cell membranes, but also in aromatic essential oils. Monitoring the level of lipid droplets in plant cells using fluorescent probes provides a simple method for screening lipid-rich varieties. We synthesized a polarity-viscosity responsive coumarin fluorescent probe, Cou-CN, which achieved sensitive detection of polarity and viscosity in dilute solution environments by constructing this simple probe with ICT and TICT properties and verifying it using Gaussian computational simulation. Cou-CN exhibited good lipid droplet illumination effects in HepG2 cells with a correlation coefficient of 0.92 compared to the commercial lipid droplet dye BODIPY. Additionally, co-staining the probe with the lipophilic commercial dye Nile Red in tobacco root stem seedling cells resulted in a high correlation coefficient of 0.9.

Indexed as

Fluorescent DyesNicotianaPlant RootsCoumarinsHep G2 CellsHumansLipid DropletsMolecular StructureOptical ImagingViscosityCoumarinsFluorescent Dyes

Identifiers

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.