ArticleActa odontologica Scandinavica2024
The effect of citric acid on mineralisation and vascular endothelial growth factor secretion from apical papilla stem cells.
Article in Acta odontologica Scandinavica, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Prolonged high-density culture with restricted medium renewal induces epithelial-like plasticity in apical papilla stem cells.Biomolecules & biomedicine · 2026Article
- Article
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7 authors.
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No grant is acknowledged in the PubMed record.
Abstract
objectiveTo investigate the influence of citric acid on the osteogenic and angiogenic potential of stem cells from apical papillae (SCAPs). MATERIALS AND
methodsStem cells from apical papillae were isolated from freshly extracted third permanent molars. These cells were treated with 20 and 100 μM citric acid. Alizarin red staining was used to evaluate mineral deposition. The secreted levels of vascular endothelial growth factor (VEGF) were assessed by ELISA on days 18, 24 and 28. Immunofluorescence analysis was performed to assess the expression of surface markers after exposure to 20 and 100 μM citric acid.
resultsDifferent mineralisation patterns were observed. Supplemented with citric acid, media showed more diffuse and less dense crystals. On day 18, most VEGF was secreted from the cells with no added citric acid. On day 24, there was a significant increase (p < 0.05) in the levels of VEGF secreted from cells treated with 20 μM citric acid. On day 28, cells from the control group did not secrete VEGF. There was a reduction in the levels of VEGF secreted by cells treated with 20 μM citric acid and a significant increase in the cells exposed to 100 μM citric acid (p < 0.05).
conclusionCitric acid can promote the differentiation of SCAPs and angiogenesis.
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