Evidence map›Paper›PMID 39350051›Full record

ArticleGenetics, selection, evolution : GSE2024

QTL analysis to identify genes involved in the trade-off between silk protein synthesis and larva-pupa transition in silkworms.

Rui Gao, Chunlin Li, Ang Zhou, Xiachao Wang, Kupeng Lu, Weidong Zuo, Hai Hu, Minjin Han, Xiaoling Tong, Fangyin Dai

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Genetics, selection, evolution : GSE, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Rui GaoState Key Laboratory of Resource Insects, Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, College of Biotechnology, Yibin Academy of Southwest University, Southwest University, Chongqing, 400715, China.
Chunlin LiState Key Laboratory of Resource Insects, Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, College of Biotechnology, Yibin Academy of Southwest University, Southwest University, Chongqing, 400715, China.
Ang ZhouState Key Laboratory of Resource Insects, Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, College of Biotechnology, Yibin Academy of Southwest University, Southwest University, Chongqing, 400715, China.
Xiachao WangState Key Laboratory of Resource Insects, Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, College of Biotechnology, Yibin Academy of Southwest University, Southwest University, Chongqing, 400715, China.
Kupeng LuState Key Laboratory of Resource Insects, Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, College of Biotechnology, Yibin Academy of Southwest University, Southwest University, Chongqing, 400715, China.
Weidong ZuoState Key Laboratory of Resource Insects, Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, College of Biotechnology, Yibin Academy of Southwest University, Southwest University, Chongqing, 400715, China.
Hai HuState Key Laboratory of Resource Insects, Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, College of Biotechnology, Yibin Academy of Southwest University, Southwest University, Chongqing, 400715, China.
Minjin HanState Key Laboratory of Resource Insects, Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, College of Biotechnology, Yibin Academy of Southwest University, Southwest University, Chongqing, 400715, China.
Xiaoling TongState Key Laboratory of Resource Insects, Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, College of Biotechnology, Yibin Academy of Southwest University, Southwest University, Chongqing, 400715, China.
Fangyin DaiState Key Laboratory of Resource Insects, Key Laboratory of Sericultural Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, College of Biotechnology, Yibin Academy of Southwest University, Southwest University, Chongqing, 400715, China. fydai@swu.edu.cn.

Funding

China Agriculture Research System of MOF and MARA CARS-18-ZJ0102Fundamental Research Funds for the Central Universities SWU-KQ22005National Natural Science Foundation of China 32330102National Natural Science Foundation of China U20A2058Natural Science Foundation of Chongqing, China cstc2021jcyj-bshX0014Natural Science Foundation of Chongqing, China cstc2021jcyj-cxtt0005
6 · The paper itself

Abstract

backgroundInsect-based food and feed are increasingly attracting attention. As a domesticated insect, the silkworm (Bombyx mori) has a highly nutritious pupa that can be easily raised in large quantities through large-scale farming, making it a highly promising source of food. The ratio of pupa to cocoon (RPC) refers to the proportion of the weight of the cocoon that is attributed to pupae, and is of significant value for edible utilization, as a higher RPC means a higher ratio of conversion of mulberry leaves to pupa. In silkworm production, there is a trade-off between RPC and cocoon shell ratiao(CSR), which refers the ratio of silk protein to the entire cocoon, during metamorphosis process. Understanding the genetic basis of this balance is crucial for breeding edible strains with a high RPC and further advancing its use as feed.

resultsUsing QTL-seq, we identified a quantitative trait locus (QTL) for the balance between RPC and CSR that is located on chromosome 11 and covers a 9,773,115-bp region. This locus is an artificial selection hot spot that contains ten non-overlapping genomic regions under selection that were involved in the domestication and genetic breeding processes. These regions include 17 genes, nine of which are highly expressed in the silk gland, which is a vital component in the trade-off between RPC and CSR. These genes are annotate with function related with epigenetic modifications and the regulation of DNA replication et al. We identified one and two single nucleotide polymorphisms (SNPs) in the exons of teh KWMTBOMO06541 and KWMTBOMO06485 genes that result in amino acid changes in the protein domains. These SNPs have been strongly selected for during the domestication process. The KWMTBOMO06485 gene encodes the Bombyx mori (Bm) tRNA methyltransferase (BmDnmt2) and its knockout results in a significant change in the trade-off between CSR and RPC in both sexes.

conclusionsTaken together, our results contribute to a better understanding of the genetic basis of RPC and CSR. The identified QTL and genes that affect RPC can be used for marker-assisted and genomic selection of silkworm strains with a high RPC. This will further enhance the production efficiency of silkworms and of closely-related insects for edible and feed purposes.

Indexed as

BombyxLarvaPupaQuantitative Trait LociSilkAnimalsInsect ProteinsPolymorphism, Single NucleotideInsect ProteinsSilk

Identifiers

PMID39350051
PMCPMC11440889

What OpenQuestion holds

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LicenceCC BY
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.