ArticleBMC genomics2024
RGeasy: a reference gene analysis tool for gene expression studies via RT-qPCR.
Article in BMC genomics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Reference gene selection for gene expression analysis in Coffea arabica L. under ABA and gibberellin treatments.Molecular biology reports · 2026Article
- Selection and Validation of Suitable Reference Genes for RT-qPCR Normalization inPlants (Basel, Switzerland) · 2026Article
- Advances in algorithms for normalizer gene selection in qRT-PCR: implications for cancer biology and precision medicine.Frontiers in genetics · 2026Review
- Optimizing reference gene selection for accurate gene expression analysis in plants.Plant signaling & behavior · 2025Review
- Identification and Validation of Tissue-Specific Housekeeping Markers for the Amazon River PrawnGenes · 2025Article
- Evaluation and validation of reference genes for RT-qPCR normalization in different sweet potato tissues.Scientific reports · 2025Article
- Selection of reference genes in Thermothelomyces fergusii: insights from genomic and expression analysis in response to different carbon sources.BMC genomics · 2025Article
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Authors and funding
7 authors.
Funding
Abstract
Gene expression through RT-qPCR can be performed by the relative quantification method, which requires the expression normalization through reference genes. Therefore, it is essential to validate, experimentally, the candidate reference genes. Thus, although there are several studies that are performed to identify the most stable reference genes, most them validate genes for very specific conditions, not exploring the whole potential of the research since not all possible combinations of treatments and/or conditions of the study are explored. For this reason, new experiments must be conducted by researchers that have interest in analyzing gene expression of treatments and/or conditions present, but not explored, in these studies. Here, we present the RGeasy tool, which aims to facilitate the selection of reference genes, allowing the user to choose genes for a greater number of combinations of treatments/conditions, compared to the ones present in the original articles, through just a few clicks. RGeasy was validated with RT-qPCR data from gene expression studies performed in two coffee species, Coffea arabica and Coffea canephora, and it can be used for any animal, plant or microorganism species. In addition to displaying a rank of the most stable reference genes for each condition or treatment, the user also has access to the primer pairs for the selected reference genes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.