Evidence map›Paper›PMID 39349658›Full record

ArticleMikrochimica acta2024

Electrochemical aptasensor for sensitive detection of staphylococcal enterotoxin type A in milk and fruit juice.

Smriti Singh, Ravi Kant Agrawal, Seema Nara

Abstract read
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In one paragraph

Article in Mikrochimica acta, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Smriti SinghDepartment of Biotechnology, Motilal Nehru National Institute of Technology Allahabad, Prayagraj, 211004, U.P., India.
Ravi Kant AgrawalBiological Product Division, ICAR-IVRI Izatnagar, Bareilly, U.P., India.
Seema NaraDepartment of Biotechnology, Motilal Nehru National Institute of Technology Allahabad, Prayagraj, 211004, U.P., India. seemanara@mnnit.ac.in.ORCID http://orcid.org/0000-0001-9858-0776

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

An aptamer-based electrochemical sensor for the sensitive detection of staphylococcal enterotoxin type A (SEA) is presented. The truncated aptamer AptSEA1.4 used in this work was screened using computational techniques, which reduced the cost of the SELEX screening process. The aptamer-SEA interactions were confirmed by employing circular dichroism (CD) and fluorescence spectroscopy. Afterwards, for developing an electrochemical aptasensor, a fabricated GNR/FTO aptasensor was prepared and characterized using scanning electron microscopy-energy-dispersive X-ray analysis (SEM-EDX), atomic force microscopy (AFM), cyclic voltammetry (CV), and square wave voltammetry (SWV). A detailed investigation of aptamer and SEA interaction in the presence of various experimental conditions was also conducted through SWV and electrochemical impedance spectroscopy (EIS). The aptamer exhibits a strong affinity for SEA, with a dissociation constant (Kd) of 19.93 nM. The aptasensor is sensitive, with a lower limit of detection of 12.44 pg mL

Indexed as

Aptamers, NucleotideBiosensing TechniquesElectrochemical TechniquesEnterotoxinsFruit and Vegetable JuicesLimit of DetectionMilkAnimalsFood ContaminationAptamers, Nucleotideenterotoxin A, StaphylococcalEnterotoxinsAptamerAptasensorElectrochemical detectionElectrochemical impedance spectroscopySquare wave voltammetryStaphylococcal enterotoxin type-ATruncation

Identifiers

PMID39349658

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.