Evidence map›Paper›PMID 39349347›Full record

ArticleJournal of pediatric surgery2024

Distraction Enterogenesis Induces Desert Hedgehog in the Lengthened Murine Colon.

Siavash Shariatzadeh, Arshia Tofang Sazi, James C Y Dunn

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of pediatric surgery, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Siavash ShariatzadehDepartment of Surgery, Stanford University, Stanford, CA, United States.
Arshia Tofang SaziDepartment of Surgery, Stanford University, Stanford, CA, United States.
James C Y DunnDepartment of Surgery, Stanford University, Stanford, CA, United States; Division of Bioengineering, Stanford University, Stanford, CA, United States. Electronic address: jdunn2@stanford.edu.

Funding

Spring Mediated EnterogenesisR01DK130972 · NIDDK · STANFORD UNIVERSITY · PI James C Dunn · 2021 to 2026
$1.8M
Nikon A1Rsi resonant spectral confocal microscopeS10OD010580 · OD · STANFORD UNIVERSITY · PI FULLER, MARGARET T · 2013 to 2013
$377k
NIDDK NIH HHS R01 DK130972NIH HHS S10 OD010580
6 · The paper itself

Abstract

backgroundDistraction enterogenesis lengthens the intestine through applied mechanical stress. The Hedgehog pathway (Hh) is responsible for intestinal tract development and directing the multi-layer patterning of the intestinal lumen. This study investigates the alteration in the principal components of this pathway in spring-mediated colonic lengthening.

methodsSamples from the murine cecal lengthening model were used to study Hh alteration during the cecal lengthening process. Primary components of this pathway were analyzed using RT-qPCR and immunostaining after 7 and 14 days of force application. The spring-mediated lengthened segments were compared to untreated control segments within each animal.

resultsThe spring-treated segments showed a 50% increase in length. There was a significant increase in the expression of the Desert Hedgehog ligand as opposed to the Sonic Hedgehog and Indian Hedgehog ligands. Additionally, the downstream targets of the pathway, Gli1, Gli2, and Gli3, were significantly overexpressed. The highest alterations in these components occurred at the earlier time point, after 7 days.

conclusionsThese findings highlight the contribution of the conserved Hedgehog developmental pathway during mechanical force-induced cecal lengthening, primarily through the Desert Hedgehog ligand. These data suggest that the Desert Hedgehog pathway may serve as therapeutic targets for intestinal regeneration.

Indexed as

CecumColonHedgehog ProteinsAnimalsKruppel-Like Transcription FactorsMaleMiceMice, Inbred C57BLNerve Tissue ProteinsSignal TransductionZinc Finger Protein GLI1Zinc Finger Protein Gli2Zinc Finger Protein Gli3Gli1 protein, mouseGli2 protein, mouseGli3 protein, mouseHedgehog ProteinsKruppel-Like Transcription FactorsNerve Tissue ProteinsZinc Finger Protein GLI1Zinc Finger Protein Gli2Zinc Finger Protein Gli3Colonic lengtheningDesert HedgehogDistraction enterogenesisEndoluminal deviceHedgehogShort bowel syndrome (SBS)

Identifiers

PMID39349347
PMCPMC12741256

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.