Evidence map›Paper›PMID 39347946›Full record

ArticlePediatric surgery international2024

Alpha-1-antitrypsin improves anastomotic healing in intestinal epithelial cells model.

Nagoud Schukfeh, Kokilavani Sivaraman, Aileen Schmidt, Gertrud Vieten, Jens Dingemann, Johannes Weidner, Ruth Olmer, Sabina Janciauskiene

Abstract read
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In one paragraph

Article in Pediatric surgery international, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Safety and Tolerance ofNutrients · 2025
    Trial
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Nagoud SchukfehDepartment of Pediatric Surgery, Hannover Medical School, Hannover, Germany. schukfeh.nagoud@mh-hannover.de.
Kokilavani SivaramanDepartment of Respiratory Medicine, Hannover Medical School, Hannover, Germany.
Aileen SchmidtBiomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), German Center for Lung Research (DZL), Hannover, Germany.
Gertrud VietenDepartment of Pediatric Surgery, Hannover Medical School, Hannover, Germany.
Jens DingemannDepartment of Pediatric Surgery, Hannover Medical School, Hannover, Germany.
Johannes WeidnerDepartment of Pediatric Surgery, Hannover Medical School, Hannover, Germany.
Ruth OlmerBiomedical Research in Endstage and Obstructive Lung Disease Hannover (BREATH), German Center for Lung Research (DZL), Hannover, Germany.
Sabina JanciauskieneDepartment of Respiratory Medicine, Hannover Medical School, Hannover, Germany.

Funding

MHH Plus Stiftung 19430068
6 · The paper itself

Abstract

purposeIntestinal anastomosis is a routine procedure in pediatric surgery, with leakage being a significant complication. Human alpha1-antitrypsin (AAT), whose physiological serum concentrations range from 0.9-2.0 mg/ml, is known to accelerate wound healing and stimulate the expression of cell proliferation-related genes. We hypothesized that AAT might enhance anastomotic healing.

methodsIn a monolayer of non-tumorigenic HIEC-6 epithelial cells derived from fetal intestine a scratch was created. Standard medium without (control) or with AAT (0.5 and 1 mg/ml) was added. Cells were observed using a Life-Cell Imaging System. Cell proliferation was assessed, and the expression of proliferation-related genes was measured by qRT-PCR.

resultsIn the presence of AAT, the scratch closed significantly faster. Cells treated with 1 mg/ml AAT showed 53% repopulation after 8 h and 97% after 18 h, while control cells showed 24% and 60% repopulation, respectively (p < 0.02). The treatment with AAT induced HIEC-6-cell proliferation and significantly increased the mRNA-expression of CDKN1A, CDKN2A, ANGPTL4, WNT3 and COL3A1 genes. AAT did not change the mRNA-expression of CXCL8 but decreased levels of IL-8 as compared to controls.

conclusionAt physiological concentrations AAT accelerates the confluence of intestinal cells and increases cell proliferation. The local administration of AAT may bear therapeutic potential to improve anastomotic healing.

Indexed as

alpha 1-AntitrypsinAnastomosis, SurgicalCell ProliferationWound HealingCells, CulturedEpithelial CellsHumansIntestinal Mucosaalpha 1-AntitrypsinAnastomosisIn vitroPediatric surgeryWound healing

Identifiers

PMID39347946

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.