Evidence map›Paper›PMID 39347036›Full record

ArticleJournal of dental sciences2024

Potential different immune phenotypes of macrophages in oral lichen planus by integrating immunofluorescence double staining and single-cell RNA sequencing.

Qianqian Zhang, Ran Zhao, Xuemin Shen, Kai Sun

Abstract read
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In one paragraph

Article in Journal of dental sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Qianqian ZhangDepartment of Oral Medicine, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Shanghai, China.
Ran ZhaoDepartment of Oral Medicine, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Shanghai, China.
Xuemin ShenDepartment of Oral Medicine, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Shanghai, China.
Kai SunDepartment of Oral Medicine, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, College of Stomatology, Shanghai Jiao Tong University, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Shanghai Key Laboratory of Stomatology, Shanghai Research Institute of Stomatology, Shanghai, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background/purpose: Oral lichen planus (OLP) is a chronic inflammatory disease with obscure etiopathogenesis. Macrophages play an important role in interaction between innate and adaptive immunity. This study aimed to investigate the macrophage phenotypes and obtain more comprehensive gene characteristics of macrophages in OLP. Materials and methods: Double cluster of differentiation (CD) 68/CD86 and CD68/CD206 immunofluorescence staining was conducted in 11 biopsy-proven OLP tissue samples and 5 health control (HC) to represent M1 and M2 macrophages, respectively. The number of positively stained cells was manually counted, and the density was calculated. Furtherly, OLP single-cell dataset GSE211630 was downloaded from Gene Expression Omnibus. Gene characteristics and functional analysis of the macrophages were elucidated. Results: In the OLP group, the densities of M1 ( Conclusion: A pro-inflammatory status of macrophages with different gene characteristics was found in the microenvironment of OLP by integrating immunofluorescence double staining and single-cell RNA sequencing, which provided a potential target for clinical treatment of OLP.

Indexed as

Immunofluorescence double stainingMacrophage phenotypesOral lichen planusSingle-cell RNA sequencing

Identifiers

PMID39347036
PMCPMC11437332

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.