Evidence map›Paper›PMID 39345619›Full record

ArticlebioRxiv : the preprint server for biology2024

Quantitative Comparison of Monomeric StayGold Variants Using Protein Nanocages in Living Cells.

Giulia Viola, Kyle A Jacobs, Joël Lemière, Matthew L Kutys, Torsten Wittmann

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Giulia ViolaDepartment of Cell and Tissue Biology, University of California, San Francisco, San Francisco, CA 94941.ORCID 0009-0000-6056-0411
Kyle A JacobsDepartment of Cell and Tissue Biology, University of California, San Francisco, San Francisco, CA 94941.ORCID 0000-0002-4411-7855
Joël LemièreDepartment of Cell and Tissue Biology, University of California, San Francisco, San Francisco, CA 94941.
Matthew L KutysDepartment of Cell and Tissue Biology, University of California, San Francisco, San Francisco, CA 94941.
Torsten WittmannDepartment of Cell and Tissue Biology, University of California, San Francisco, San Francisco, CA 94941.ORCID 0000-0001-9134-691X

Funding

Quantitative analysis and manipulation of chromosome dynamics in cancer organoidsR61CA278518 · NCI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Torsten Wittmann · 2024 to 2026
$680k
Wide-field super-resolution spinning disk confocal microscopeS10OD028611 · OD · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI WITTMANN, TORSTEN · 2020 to 2020
$600k
Spinning disk confocal / FRAP microscope for quantitative live cell imagingS10RR026758 · NCRR · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI WITTMANN, TORSTEN · 2010 to 2010
$500k
NCI NIH HHS R61 CA278518NCRR NIH HHS S10 RR026758NIH HHS S10 OD028611
6 · The paper itself

Abstract

To standardize comparison of fluorescent proteins and independently determine which monomeric StayGold variant is best for live microscopy, we analyzed fluorescent protein tagged I3-01 peptides that self-assemble into stable sixty subunit dodecahedrons inside live cells. We find mStayGold is 3-fold brighter and 3-fold more photostable compared with EGFP and superior to other monomeric variants in mammalian cytoplasm. In addition, analysis of intracellular nanocage diffusion confirms the monomeric nature of mStayGold.

Identifiers

PMID39345619
PMCPMC11429717

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.