Evidence map›Paper›PMID 39345518›Full record

ArticlebioRxiv : the preprint server for biology2024

Human Neural Organoid Microphysiological Systems Show the Building Blocks Necessary for Basic Learning and Memory.

Dowlette-Mary Alam El Din, Leah Moenkemoeller, Alon Loeffler, Forough Habibollahi, Jack Schenkman, Amitav Mitra, Tjitse van der Molen, Lixuan Ding, Jason Laird, Maren Schenke and 4 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

14 authors.

Dowlette-Mary Alam El DinCenter for Alternatives to Animal Testing (CAAT), Johns Hopkins University, Baltimore, MD.
Leah MoenkemoellerCenter for Alternatives to Animal Testing (CAAT), Johns Hopkins University, Baltimore, MD.
Alon LoefflerCortical Labs Pty Ltd; Melbourne, Australia.
Forough HabibollahiCortical Labs Pty Ltd; Melbourne, Australia.
Jack SchenkmanDepartment of Electrical and Computer Engineering, Princeton University, Princeton NJ.
Amitav MitraDepartment of Physics and Astronomy, Johns Hopkins University, Baltimore MD.
Tjitse van der MolenNeuroscience Research Institute, University of California Santa Barbara, Santa Barbara, CA.
Lixuan DingCenter for Alternatives to Animal Testing (CAAT), Johns Hopkins University, Baltimore, MD.
Jason LairdCenter for Alternatives to Animal Testing (CAAT), Johns Hopkins University, Baltimore, MD.
Maren SchenkeCenter for Alternatives to Animal Testing (CAAT), Johns Hopkins University, Baltimore, MD.
Erik C JohnsonResearch and Exploratory Development Department, Johns Hopkins University Applied Physics Laboratory, Laurel, MD, United States.ORCID 0000-0002-7397-8531
Brett J KaganCortical Labs Pty Ltd; Melbourne, Australia.ORCID 0000-0003-3355-7553
Thomas HartungCenter for Alternatives to Animal Testing (CAAT), Johns Hopkins University, Baltimore, MD.
Lena SmirnovaCenter for Alternatives to Animal Testing (CAAT), Johns Hopkins University, Baltimore, MD.

Funding

Traning Program in Environmental Health SciencesT32ES007141 · NIEHS · JOHNS HOPKINS UNIVERSITY · PI Marsha Wills-Karp · 1985 to 2026
$22.2M
Olympus FluoView3000RS Confocal MicroscopeS10OD025244 · OD · JOHNS HOPKINS UNIVERSITY · PI O'ROURKE, BRIAN · 2018 to 2018
$448k
NIEHS NIH HHS T32 ES007141NIH HHS S10 OD025244
6 · The paper itself

Abstract

Brain Microphysiological Systems including neural organoids derived from human induced pluripotent stem cells offer a unique lens to study the intricate workings of the human brain. This paper investigates the foundational elements of learning and memory in neural organoids, also known as Organoid Intelligence by quantifying immediate early gene expression, synaptic plasticity, neuronal network dynamics, and criticality to demonstrate the utility of these organoids in basic science research. Neural organoids showed synapse formation, glutamatergic and GABAergic receptor expression, immediate early gene expression basally and evoked, functional connectivity, criticality, and synaptic plasticity in response to theta-burst stimulation. In addition, pharmacological interventions on GABAergic and glutamatergic receptors, and input specific theta-burst stimulation further shed light on the capacity of neural organoids to mirror synaptic modulation and short-term potentiation, demonstrating their potential as tools for studying neurophysiological and neurological processes and informing therapeutic strategies for diseases.

Identifiers

PMID39345518
PMCPMC11429697

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.