Evidence map›Paper›PMID 39345501›Full record

ArticlebioRxiv : the preprint server for biology2024

Design and immunogenicity of an HIV-1 clade C pediatric envelope glycoprotein stabilized by multiple platforms.

Sanjeev Kumar, Iván Del Moral-Sánchez, Swarandeep Singh, Maddy L Newby, Joel D Allen, Tom P L Bijl, Yog Vaghani, Liang Jing, Eric A Ortlund, Max Crispin and 3 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

13 authors.

Sanjeev KumarDepartment of Medical Microbiology and Infection Prevention, Amsterdam UMC, University of Amsterdam, Amsterdam, 1105AZ, The Netherlands.ORCID 0000-0002-8181-3999
Iván Del Moral-SánchezDepartment of Medical Microbiology and Infection Prevention, Amsterdam UMC, University of Amsterdam, Amsterdam, 1105AZ, The Netherlands.ORCID 0000-0002-6356-1484
Swarandeep SinghDepartment of Biochemistry, All India Institute of Medical Sciences, New Delhi, 110029, India.ORCID 0000-0003-1186-1327
Maddy L NewbySchool of Biological Sciences, University of Southampton, Southampton, SO17 1BJ, UK.
Joel D AllenSchool of Biological Sciences, University of Southampton, Southampton, SO17 1BJ, UK.ORCID 0000-0003-2547-968X
Tom P L BijlDepartment of Medical Microbiology and Infection Prevention, Amsterdam UMC, University of Amsterdam, Amsterdam, 1105AZ, The Netherlands.
Yog VaghaniDepartment of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA.
Liang JingDepartment of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA.
Eric A OrtlundDepartment of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA.ORCID 0000-0001-8855-3029
Max CrispinSchool of Biological Sciences, University of Southampton, Southampton, SO17 1BJ, UK.ORCID 0000-0002-1072-2694
Anamika PatelDepartment of Biochemistry, Emory University School of Medicine, Atlanta, GA 30322, USA.
Rogier W SandersDepartment of Medical Microbiology and Infection Prevention, Amsterdam UMC, University of Amsterdam, Amsterdam, 1105AZ, The Netherlands.
Kalpana LuthraDepartment of Biochemistry, All India Institute of Medical Sciences, New Delhi, 110029, India.ORCID 0000-0002-7801-2867

Funding

Structural studies of native-like SOSIP trimersP01AI110657 · NIAID · WEILL MEDICAL COLL OF CORNELL UNIV · PI MOORE, JOHN P · 2015 to 2024
$35.4M
NIAID NIH HHS P01 AI110657
6 · The paper itself

Abstract

Various design platforms are available to stabilize soluble HIV-1 envelope (Env) trimers, which can be used as antigenic baits and vaccine antigens. However, stabilizing HIV-1 clade C trimers can be challenging. Here, we stabilized an HIV-1 clade C trimer based on an Env isolated from a pediatric elite-neutralizer (AIIMS_329) using multiple platforms, including SOSIP.v8.2, ferritin nanoparticles (NP) and an I53-50 two-component NP, followed by characterization of their biophysical, antigenic, and immunogenic properties. The stabilized 329 Envs showed binding affinity to trimer-specific HIV-1 broadly neutralizing antibodies (bnAbs), with negligible binding to non-neutralizing antibodies (non-nAbs). Negative-stain electron microscopy (nsEM) confirmed the native-like conformation of the Envs. Multimerization of 329 SOSIP.v8.2 on ferritin and two-component I53-50 NPs improved the overall affinity to HIV-1 bnAbs and immunogenicity in rabbits. These stabilized HIV-1 clade C 329 Envs demonstrate the potential to be used as antigenic baits and as components of multivalent vaccine candidates in future.

Indexed as

broadly neutralizing antibodiesferritin nanoparticleHIV-1CnsEMpediatric elite-neutralizerrabbit immunizationSOSIP Env trimertwo-component nanoparticle

Identifiers

PMID39345501
PMCPMC11429718

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.