Evidence map›Paper›PMID 39345499›Full record

ArticlebioRxiv : the preprint server for biology2024

Intravital imaging of pulmonary lymphatics in inflammation and metastatic cancer.

Simon J Cleary, Longhui Qiu, Yurim Seo, Peter Baluk, Dan Liu, Nina K Serwas, Jason G Cyster, Donald M McDonald, Matthew F Krummel, Mark R Looney

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors.

Simon J ClearyDepartment of Medicine, University of California, San Francisco (UCSF), CA, USA.ORCID 0000-0001-5573-6363
Longhui QiuDepartment of Medicine, University of California, San Francisco (UCSF), CA, USA.ORCID 0000-0003-3122-9679
Yurim SeoDepartment of Medicine, University of California, San Francisco (UCSF), CA, USA.
Peter BalukDepartment of Anatomy, Cardiovascular Research Institute, and Helen Diller Family Comprehensive Cancer Center, UCSF, CA, USA.
Dan LiuHoward Hughes Medical Institute and Department of Microbiology and Immunology, UCSF, CA, USA.
Nina K SerwasDepartment of Pathology, UCSF, CA, USA.
Jason G CysterHoward Hughes Medical Institute and Department of Microbiology and Immunology, UCSF, CA, USA.
Donald M McDonaldDepartment of Anatomy, Cardiovascular Research Institute, and Helen Diller Family Comprehensive Cancer Center, UCSF, CA, USA.
Matthew F KrummelDepartment of Pathology, UCSF, CA, USA.
Mark R LooneyDepartment of Medicine, University of California, San Francisco (UCSF), CA, USA.ORCID 0000-0003-0241-9190

Funding

ENDOTHELIAL PERMEABILITY IN AIRWAY ANGIOGENESISR01HL059157 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI MCDONALD, DONALD M · 1998 to 2015
$5.5M
Immunobiology of the normal and injured lungR35HL161241 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI MARK ROBERTS LOONEY · 2022 to 2026
$4.2M
Immunomodulation by splenic megakaryocytes and platelets in sepsisR01AI165919 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI LOONEY, MARK ROBERTS · 2022 to 2025
$3.2M
Mechanisms and pathogenicity of SARS-CoV-2-induced neutrophil extracellular trapsR01AI160167 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI LOONEY, MARK ROBERTS · 2021 to 2025
$3.1M
Angiopoietin/Tie signaling regulation of vascular leakage in lung inflammationR01HL143896 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI MCDONALD, DONALD M · 2018 to 2021
$2.5M
Mechanisms, consequences, and reversal of abnormalities in lung lymphaticsR01HL127402 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI MCDONALD, DONALD M · 2015 to 2018
$2.2M
NHLBI NIH HHS R01 HL059157NHLBI NIH HHS R01 HL127402NHLBI NIH HHS R01 HL143896NHLBI NIH HHS R35 HL161241NIAID NIH HHS R01 AI160167NIAID NIH HHS R01 AI165919
6 · The paper itself

Abstract

Intravital microscopy has enabled the study of immune dynamics in the pulmonary microvasculature, but many key events remain unseen because they occur in deeper lung regions. We therefore developed a technique for stabilized intravital imaging of bronchovascular cuffs and collecting lymphatics surrounding pulmonary veins in mice. Intravital imaging of pulmonary lymphatics revealed ventilation-dependence of steady-state lung lymph flow and ventilation-independent lymph flow during inflammation. We imaged the rapid exodus of migratory dendritic cells through lung lymphatics following inflammation and measured effects of pharmacologic and genetic interventions targeting chemokine signaling. Intravital imaging also captured lymphatic immune surveillance of lung-metastatic cancers and lymphatic metastasis of cancer cells. To our knowledge, this is the first imaging of lymph flow and leukocyte migration through intact pulmonary lymphatics. This approach will enable studies of protective and maladaptive processes unfolding within the lungs and in other previously inaccessible locations.

Indexed as

cancerdendritic cellsinflammationintravital microscopyleukocyte traffickinglungLymphaticsmetastasispulmonary

Identifiers

PMID39345499
PMCPMC11430110

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.