Evidence map›Paper›PMID 39345383›Full record

ArticlebioRxiv : the preprint server for biology2024

Structure-guided engineering of type I-F CASTs for targeted gene insertion in human cells.

George D Lampe, Ashley R Liang, Dennis J Zhang, Israel S Fernández, Samuel H Sternberg

Abstract readPreprint
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors.

George D LampeDepartment of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA.ORCID 0000-0002-2873-2372
Ashley R LiangDepartment of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA.ORCID 0009-0004-1465-9743
Dennis J ZhangDepartment of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA.ORCID 0000-0002-6871-9842
Israel S FernándezIkerbasque, Basque Foundation for Science, Bilbao, Spain.ORCID 0000-0001-7218-1603
Samuel H SternbergDepartment of Biochemistry and Molecular Biophysics, Columbia University, New York, NY, USA.ORCID 0000-0001-8240-9114

Funding

NCCAT: National Center for CryoEM Access and Training- Supplement for Windows 10 and FFIU24GM129539 · NIGMS · NEW YORK STRUCTURAL BIOLOGY CENTER · PI DE MARCO, ALEX, KIEFT, JEFFREY S · 2018 to 2023
$53.9M
Center for Genomic Editing and Recording: Development and Application of Next-Generation Genome and Epigenome Editing Methods to Advance the Study and Treatment of Human DiseaseRM1HG009490 · NHGRI · WHITEHEAD INSTITUTE FOR BIOMEDICAL RES · PI Brittany S. Adamson, Martin Joseph Ankrah Aryee · 2017 to 2026
$22.7M
ePACE: automation platforms for adaptable and scalable continuous evolution of biomolecules with therapeutic potentialR01EB027793 · NIBIB · BROAD INSTITUTE, INC. · PI Ahmad Samir Khalil, DAVID R LIU · 2019 to 2026
$6.1M
A high-performance and versatile technology for precision microbiome engineeringR01EB031935 · NIBIB · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI Samuel Henry Sternberg, Harris H Wang · 2021 to 2026
$3.3M
Leveraging Programmable Integrases for Human Genome EngineeringDP2HG011650 · NHGRI · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI STERNBERG, SAMUEL HENRY · 2020 to 2020
$2.4M
Impact of CRISPR-associated transposons on anti-phage immunity in Vibrio choleraeR21AI168976 · NIAID · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI STERNBERG, SAMUEL HENRY · 2022 to 2023
$446k
NHGRI NIH HHS DP2 HG011650NHGRI NIH HHS RM1 HG009490NIAID NIH HHS R21 AI168976NIBIB NIH HHS R01 EB027793NIBIB NIH HHS R01 EB031935NIGMS NIH HHS U24 GM129539
6 · The paper itself

Abstract

Conventional genome editing tools rely on DNA double-strand breaks (DSBs) and host recombination proteins to achieve large insertions, resulting in a heterogeneous mixture of undesirable editing outcomes. We recently leveraged a type I-F CRISPR-associated transposase (CAST) from the

Identifiers

PMID39345383
PMCPMC11429998

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.