Evidence map›Paper›PMID 39343750›Full record

ArticleZhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences2024

[Construction of HEK293T cell line stably expressing TRPM2 channel based on PiggyBac transposition system and its application in drug screening for cerebral ischemia and other diseases].

Kaiyue Ying, Ning Hua, Yanping Luo, Xingyu Liu, Min Liu, Wei Yang

Abstract readEnglish Abstract
PubMed Full text at PMC Publisher
In one paragraph

Article in Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Kaiyue YingDepartment of Biophysics, School of Basic Medical Sciences, Zhejiang University, Hangzhou 310058, China. 22160383@zju.edu.cn.
Ning HuaDepartment of Biophysics, School of Basic Medical Sciences, Zhejiang University, Hangzhou 310058, China.
Yanping LuoDepartment of Biophysics, School of Basic Medical Sciences, Zhejiang University, Hangzhou 310058, China.
Xingyu LiuDepartment of Biophysics, School of Basic Medical Sciences, Zhejiang University, Hangzhou 310058, China.
Min LiuDepartment of Biophysics, School of Basic Medical Sciences, Zhejiang University, Hangzhou 310058, China.
Wei YangDepartment of Biophysics, School of Basic Medical Sciences, Zhejiang University, Hangzhou 310058, China. yangwei@zju.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivesTo establish a cell line stably expressing the transient receptor potential melastatin 2 (TRPM2) channel for screening TRPM2 inhibitors based on PiggyBac transposition system.

methodsA plasmid PiggyBac-human TRPM2 (pPB-hTRPM2) eukaryotic expression vector was constructed using PiggyBac transposition system. The plasmid and a helper plasmid were co-transfected into HEK293T cells to express TRPM2, which was identified by fluorescence and patch-clamp assays. The high throughput screening performance was assessed with the

resultsThe HEK293T cells transfected with pPB-hTRPM2-EGFP showed high

conclusionsA stable TRPM2 gene expressing cell line has been successfully established using PiggyBac gene editing in this study. TRPM2 channel inhibitors were screened through calcium imaging and patch clamp techniques, and an inhibitor compound 6 was identified. This compound can alleviate cell damage after OGD/R by reducing cellular ROS levels and has a protective effect against cerebral ischemia-reperfusion injury in mice.

Indexed as

Brain IschemiaTRPM Cation ChannelsAnimalsCalciumDrug Evaluation, PreclinicalGenetic VectorsHEK293 CellsHumansMiceNeuroprotective AgentsPatch-Clamp TechniquesPlasmidsReactive Oxygen SpeciesTransfectionCalciumNeuroprotective AgentsReactive Oxygen SpeciesTRPM2 protein, humanTRPM Cation ChannelsHigh-throughput screeningMiceOxygen-glucose deprivation/reperfusion injuryPatch clampPiggyBac transposition systemTransient middle cerebral artery occlusionTransient receptor potential melastatin 2

Identifiers

PMID39343750
PMCPMC11528149

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.