Evidence map›Paper›PMID 39342331›Full record

ArticleAnnals of clinical microbiology and antimicrobials2024

Deciphering the possible role of MmpL7 efflux pump in SQ109 resistance in Mycobacterium tuberculosis.

Wei Jing, Fuzhen Zhang, Yuanyuan Shang, Wenhui Shi, Cong Yao, Xuxia Zhang, Naihui Chu, Jie Lu, Jinfeng Yuan

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In one paragraph

Article in Annals of clinical microbiology and antimicrobials, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Wei Jing *Department of Tuberculosis, Beijing Chest Hospital, Capital Medical University, Beijing Thoracic Tumor Research Institute, No. 9, Beiguan Street, Tongzhou District, Beijing, 101149, PR China.
Fuzhen Zhang *Department of Bacteriology and Immunology, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis & Thoracic Tumor Research Institute, No. 9, Beiguan Street, Tongzhou District, Beijing, 101149, PR China.
Yuanyuan Shang *Department of Bacteriology and Immunology, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis & Thoracic Tumor Research Institute, No. 9, Beiguan Street, Tongzhou District, Beijing, 101149, PR China.
Wenhui ShiDepartment of Tuberculosis, Beijing Chest Hospital, Capital Medical University, Beijing Thoracic Tumor Research Institute, No. 9, Beiguan Street, Tongzhou District, Beijing, 101149, PR China.
Cong YaoDepartment of Bacteriology and Immunology, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis & Thoracic Tumor Research Institute, No. 9, Beiguan Street, Tongzhou District, Beijing, 101149, PR China.
Xuxia ZhangDepartment of Bacteriology and Immunology, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis & Thoracic Tumor Research Institute, No. 9, Beiguan Street, Tongzhou District, Beijing, 101149, PR China.
Naihui ChuDepartment of Tuberculosis, Beijing Chest Hospital, Capital Medical University, Beijing Thoracic Tumor Research Institute, No. 9, Beiguan Street, Tongzhou District, Beijing, 101149, PR China. chunaihui1994@sina.com.
Jie LuBeijing Key Laboratory for Pediatric Diseases of Otolaryngology, Head and Neck Surgery, Beijing Pediatric Research Institute, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, 101149, PR China. lujiebch@163.com.
Jinfeng YuanDepartment of Bacteriology and Immunology, Beijing Chest Hospital, Capital Medical University, Beijing Tuberculosis & Thoracic Tumor Research Institute, No. 9, Beiguan Street, Tongzhou District, Beijing, 101149, PR China. yuanjinfeng0920@163.com.

Funding

Beijing Natural Science Foundation (Youth Program) No.7224324"Young talents" program of Beijing hospitals Authority QML20231606
6 · The paper itself

Abstract

backgroundSQ109 is a promising candidate drug for the treatment of patients with drug-resistant tuberculosis (DR-TB). The purpose of this study was to investigate the activity of SQ109 against clinical isolates of Mycobacterium tuberculosis (MTB) from patients with multidrug-resistant TB (MDR-TB) and pre-extensively drug-resistant TB (pre-XDR-TB), and to explore new drug-resistant mechanisms of SQ109.

methodsWe evaluated the in vitro activity of SQ109 against clinical isolates from patients with MDR-TB and pre-XDR-TB using minimal inhibitory concentration (MIC) assay. The drug-resistant gene, mmpL3 of SQ109-resistant strains was sequenced, and a quantitative real-time PCR assay was used to analyze 28 efflux pump genes in SQ109-resistant strains without mmpL3 mutations. The role of candidate efflux pumps mmpL5 and mmpL7 on the MIC of SQ109 was evaluated using recombinantly cloned MmpL5 and MmpL7 expressed in Mycobacterium smegmatis.

resultsThe MIC

conclusionsOur data demonstrated that SQ109 exhibited excellent levels of in vitro activity against MTB. MmpL7 may be a potential gene for MTB resistance to SQ109, providing a useful target for detecting SQ109 resistance in MTB.

Indexed as

Antitubercular AgentsBacterial ProteinsMembrane Transport ProteinsMicrobial Sensitivity TestsMycobacterium tuberculosisTuberculosis, Multidrug-ResistantAdamantaneDrug Resistance, Multiple, BacterialEthylenediaminesHumansMutationMycobacterium smegmatisAdamantaneAntitubercular AgentsBacterial ProteinsEthylenediaminesMembrane Transport ProteinsN-geranyl-N'-(2-adamantyl)ethane-1,2-diamineMmpL7Mycobacterium tuberculosisSQ109Susceptibility

Identifiers

PMID39342331
PMCPMC11439249

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.