Evidence map›Paper›PMID 39340535›Full record

ArticlePlanta2024

Regulatory mechanisms of miR171d-SCL6 module in the rooting process of Acer rubrum L.

Huiju Li, Jiayu Yu, Jiaming Qin, Hewen Zhao, Kezhong Zhang, Wei Ge

Abstract read
PubMed Publisher
In one paragraph

Article in Planta, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. MsmiR171 targetsHorticulture research · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Huiju Li *College of Landscape Architecture, Beijing University of Agriculture, Beijing, 102206, China.
Jiayu Yu *College of Landscape Architecture, Beijing University of Agriculture, Beijing, 102206, China.
Jiaming QinCollege of Landscape Architecture, Beijing University of Agriculture, Beijing, 102206, China.
Hewen ZhaoCollege of Landscape Architecture, Beijing University of Agriculture, Beijing, 102206, China.
Kezhong ZhangCollege of Landscape Architecture, Beijing University of Agriculture, Beijing, 102206, China. kezhong.zhang@bua.edu.cn.ORCID http://orcid.org/0000-0002-0234-3547
Wei GeCollege of Landscape Architecture, Beijing University of Agriculture, Beijing, 102206, China. wei.ge@bua.edu.cn.

Funding

Beijing Innovation Consortium of Agriculture Research System No. BAIC09-2022The Project of Cultivation for Young Top-notch Talents of Beijing Municipal Institutions No. BPHR202203100
6 · The paper itself

Abstract

MAIN

conclusionMiR171d and SCL6 are induced by the plant hormone auxin. MiR171d negatively regulates the expression of SCL6, thereby regulating the growth and development of plant adventitious roots. Under natural conditions, it is difficult to induce rooting in the process of propagating Acer rubrum L. via branches, which seriously limits its wide application in landscaping construction. In this study, the expression of Ar-miR171d was downregulated and the expression of ArSCL6 was upregulated after 300 mg/L indole-3-butyric acid (IBA) treatment. The transient interaction of Ar-miR171d and ArSCL6 in tobacco cells further confirmed their cleavage activity. Transgenic function verification confirmed that OE-Ar-miR171d inhibited adventitious root (AR) development, while OE-ArSCL6 promoted AR development. Tissue-specific expression verification of the ArSCL6 promoter demonstrated that it was specifically expressed in the plant root and leaf organs. Subcellular localization and transcriptional activation assays revealed that both ArSCL6 and ArbHLH089 were located in the nucleus and exhibited transcriptional activation activity. The interaction between the two was verified by bimolecular fluorescence complementarity (BIFC) experiments. These results help elucidate the regulatory mechanisms of the Ar-miR171d-ArSCL6 module during the propagation of A. rubrum and provide a molecular basis for the rooting of branches.

Indexed as

AcerGene Expression Regulation, PlantMicroRNAsPlant RootsIndoleacetic AcidsIndolesNicotianaPlant Growth RegulatorsPlant ProteinsPlants, Genetically ModifiedPromoter Regions, GeneticIndoleacetic Acidsindolebutyric acidIndolesMicroRNAsPlant Growth RegulatorsPlant ProteinsAdventitious rootsAuxinMiR171dMolecular mechanismsPropagationSCL6Subcellular localization

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.