Multiplex PCR Approach for Rapid African Swine Fever Virus Genotyping.

Matthias Licheri, Manon Flore Licheri, Kemal Mehinagic, Emilia Radulovic, Nicolas Ruggli, Ronald Dijkman

Abstract read

In one paragraph

Article in Viruses, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

Full-Length ASFV B646L Gene Sequencing by Nanopore Offers a Simple and Rapid Approach for Identifying ASFV Genotypes.Viruses · 2024
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Matthias LicheriInstitute for Infectious Diseases, University of Bern, 3001 Bern, Switzerland.

Manon Flore LicheriInstitute for Infectious Diseases, University of Bern, 3001 Bern, Switzerland.

Kemal MehinagicGraduate School for Cellular and Biomedical Sciences, University of Bern, 3012 Bern, Switzerland.ORCID 0000-0003-2192-0028

Emilia RadulovicGraduate School for Cellular and Biomedical Sciences, University of Bern, 3012 Bern, Switzerland.

Nicolas RuggliInstitute of Virology and Immunology IVI, 3147 Mittelhäusern, Switzerland.ORCID 0000-0002-7679-6290

Ronald DijkmanInstitute for Infectious Diseases, University of Bern, 3001 Bern, Switzerland.ORCID 0000-0003-0320-2743

Funding

Federal Food Safety and Veterinary Office project 1.19.02Federal Food Safety and Veterinary Office project 1.20.02

6 · The paper itself

Abstract

African swine fever virus (ASFV) has been spreading through Europe, Asia, and the Caribbean after its introduction in Georgia in 2007 and, due to its particularly high mortality rate, poses a continuous threat to the pig industry. The golden standard to trace back the ASFV is whole genome sequencing, but it is a cost and time-intensive methodology. A more efficient way of tracing the virus is to amplify only specific genomic regions relevant for genotyping. This is mainly accomplished by amplifying single amplicons by PCR followed by Sanger sequencing. To reduce costs and processivity time, we evaluated a multiplex PCR based on the four primer sets routinely used for ASFV genotyping (

Indexed as

African Swine FeverAfrican Swine Fever VirusGenotypeGenotyping TechniquesMultiplex Polymerase Chain ReactionAnimalsDNA PrimersDNA, ViralGenome, ViralSwineDNA PrimersDNA, ViralAfrican swine fever virusCVRECO1genotypingmultiplex PCRnanopore sequencingp54p72

Identifiers

PMID39339936

DOI10.3390/v16091460

PMCPMC11437429

What OpenQuestion holds

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LicenceCC BY

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.