Evidence map›Paper›PMID 39339924›Full record

ArticleViruses2024

Structural Heterogeneity of the Rabies Virus Virion.

Xiaoying Cai, Kang Zhou, Ana Lucia Alvarez-Cabrera, Zhu Si, Hui Wang, Yao He, Cally Li, Z Hong Zhou

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Viruses, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Xiaoying CaiDepartment of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles (UCLA), Los Angeles, CA 90095-1489, USA.ORCID 0000-0001-6694-2154
Kang ZhouThe California NanoSystems Institute (CNSI), University of California, Los Angeles (UCLA), Los Angeles, CA 90095-7364, USA.ORCID 0000-0002-3034-2808
Ana Lucia Alvarez-CabreraDepartment of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles (UCLA), Los Angeles, CA 90095-1489, USA.ORCID 0000-0001-7186-9393
Zhu SiDepartment of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles (UCLA), Los Angeles, CA 90095-1489, USA.
Hui WangDepartment of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles (UCLA), Los Angeles, CA 90095-1489, USA.ORCID 0000-0002-9922-7170
Yao HeDepartment of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles (UCLA), Los Angeles, CA 90095-1489, USA.
Cally LiThe California NanoSystems Institute (CNSI), University of California, Los Angeles (UCLA), Los Angeles, CA 90095-7364, USA.
Z Hong ZhouDepartment of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles (UCLA), Los Angeles, CA 90095-1489, USA.ORCID 0000-0002-8373-4717

Funding

Genome Structure, Transcription and Packaging of dsRNA VirusesR01AI094386 · NIAID · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI Z Hong ZHOU · 2012 to 2026
$6.0M
High-Resolution CryoEM Reconstruction of Large ComplexesR01GM071940 · NIGMS · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI ZHOU, Z HONG · 2006 to 2025
$5.0M
Study of arenavirus assemblyR01AI171426 · NIAID · GEORGIA STATE UNIVERSITY · PI MING LUO, Z Hong ZHOU · 2022 to 2026
$2.7M
Direct Detection Device for atomic resolution cryoEM of macromolecular complexesS10OD018111 · OD · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ZHOU, Z HONG · 2014 to 2014
$598k
NIAID NIH HHS R01 AI094386NIAID NIH HHS R01 AI171426NIGMS NIH HHS R01 GM071940NIH HHS R01AI094386NIH HHS R01AI141726NIH HHS R01GM071940NIH HHS S10 OD018111
6 · The paper itself

Abstract

Rabies virus (RABV) is among the first recognized viruses of public health concern and has historically contributed to the development of viral vaccines. Despite these significances, the three-dimensional structure of the RABV virion remains unknown due to the challenges in isolating structurally homogenous virion samples in sufficient quantities needed for structural investigation. Here, by combining the capabilities of cryogenic electron tomography (cryoET) and microscopy (cryoEM), we determined the three-dimensional structure of the wild-type RABV virion. Tomograms of RABV virions reveal a high level of structural heterogeneity among the bullet-shaped virion particles encompassing the glycoprotein (G) trimer-decorated envelope and the nucleocapsid composed of RNA, nucleoprotein (N), and matrix protein (M). The structure of the trunk region of the virion was determined by cryoEM helical reconstruction, revealing a one-start N-RNA helix bound by a single layer of M proteins at an N:M ratio of 1. The N-M interaction differs from that in fellow rhabdovirus vesicular stomatitis virus (VSV), which features two layers of M stabilizing the N-RNA helix at an M:N ratio of 2. These differences in both M-N stoichiometry and binding allow RABV to flex its N-RNA helix more freely and point to different mechanisms of viral assembly between these two bullet-shaped rhabdoviruses.

Indexed as

Cryoelectron MicroscopyRabies virusVirionAnimalsElectron Microscope TomographyModels, MolecularNucleocapsidRabiesRNA, ViralViral Matrix ProteinsRNA, ViralViral Matrix Proteinscryogenic electron microscopycryogenic electron tomographydynamicsflexibilityrabies virusrhabdoviruseswild type

Identifiers

PMID39339924
PMCPMC11437398

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.