ArticleViruses2024
ddPCR for the Detection and Absolute Quantification of Oropouche Virus.
Article in Viruses, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
What it found
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The trial behind it
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Who cites it
4 citing papers in PubMed.
- Molecular and Serological Evidence of Oropouche Virus Circulation in Asymptomatic Blood Donors During the 2023-2024 Outbreak in Manaus, Brazil.The Journal of infectious diseases · 2026Article
- Detectability of Pathogenic RNA Virus Families in Different Body Sites: A Scoping Review.Viruses · 2026Article
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Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
backgroundOropouche virus (OROV) is a segmented RNA virus belonging to the genus
methodsThe ddPCR reaction was assessed as duplex assay using the human housekeeping gene
resultsThe LoD of the ddPCR performed using 10-fold serial dilution of OROV detected up to 1 cp/µL in all the biological matrices. Compared to the routine molecular diagnostics, the ddPCR assay showed 100% sensitivity for whole blood and serum and 75% for urine, highlighting higher positive rate of ddPCR.
conclusionWe have established a quantitative RNA detection method of OROV with high sensitivity and specificity based on ddPCR. This test is capable of quantitatively monitoring the viral load of OROV and can contribute, in addition to laboratory diagnosis, to shed light on the pathogenesis, filling in the knowledge gaps of this neglected disease and to the vector control programs.
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