Evidence map›Paper›PMID 39338884›Full record

ArticleSensors (Basel, Switzerland)2024

A Photoelectrochemical Biosensor Mediated by CRISPR/Cas13a for Direct and Specific Detection of MiRNA-21.

Yang Zhang, Pei Miao, Jingyuan Wang, Yan Sun, Jing Zhang, Bin Wang, Mei Yan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Sensors (Basel, Switzerland), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Yang ZhangSchool of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
Pei MiaoSchool of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
Jingyuan WangSchool of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
Yan SunSchool of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
Jing ZhangSchool of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
Bin WangSchool of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
Mei YanSchool of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.

Funding

Major Scientific and Technological Innovation Project of Shandong Province 2021CXGC010603National Natural Science Foundation of China 52173168Natural Science Foundation of Shandong Province ZR2022MB072Science and Technology Program of University of Jinan XKY22210Shandong Medical Association Clinical Research Fund - Qilu Special Project YXH2022ZX02118Taishan Scholars Program project TSQN tstp20221131the second hospital of Shandong University Priya Fund 2022YP21
6 · The paper itself

Abstract

Direct detection of miRNA is currently limited by the complex amplification and reverse transcription processes of existing methods, leading to low sensitivity and high operational demands. Herein, we developed a CRISPR/Cas13a-mediated photoelectrochemical (PEC) biosensing platform for direct and sensitive detection of miRNA-21. The direct and specific recognition of target miRNA-21 by crRNA-21 eliminates the need for pre-amplification and reverse transcription of miRNA-21, thereby preventing signal distortion and enhancing the sensitivity and precision of target detection. When crRNA-21 binds to miRNA-21, it activates the trans-cleavage activity of CRISPR/Cas13a, leading to the non-specific cleavage of biotin-modified DNA with uracil bases (biotin-rU-DNA). This cleavage prevents the biotin-rU-DNA from being immobilized on the electrode surface. As a result, streptavidin cannot attach to the electrode via specific biotin binding, reducing spatial resistance and causing a positively correlated increase in the photocurrent response. This Cas-PEC biosensor has good analytical capabilities, linear responses between 10 fM and 10 nM, a minimum detection limit of 9 fM, and an excellent recovery rate in the analysis of real human serum samples. This work presented an innovative solution for detecting other biomarkers in bioanalysis and clinical diagnostics.

Indexed as

Biosensing TechniquesCRISPR-Cas SystemsElectrochemical TechniquesMicroRNAsBiotinDNAElectrodesHumansLimit of DetectionBiotinDNAMicroRNAsMIRN21 microRNA, humanCRISPR/Cas13adirect recognitionmiRNA-21 detectionnucleic acid signal amplificationphotoelectrochemical biosensor

Identifiers

PMID39338884
PMCPMC11436169

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.