Evidence map›Paper›PMID 39337470›Full record

ArticleInternational journal of molecular sciences2024

Characterizing the Cell-Free Transcriptome in a Humanized Diffuse Large B-Cell Lymphoma Patient-Derived Tumor Xenograft Model for RNA-Based Liquid Biopsy in a Preclinical Setting.

Philippe Decruyenaere, Willem Daneels, Annelien Morlion, Kimberly Verniers, Jasper Anckaert, Jan Tavernier, Fritz Offner, Jo Vandesompele

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Philippe DecruyenaereDepartment of Hematology, Ghent University Hospital, 9000 Ghent, Belgium.ORCID 0000-0001-7118-8344
Willem DaneelsDepartment of Hematology, Ghent University Hospital, 9000 Ghent, Belgium.ORCID 0000-0002-5025-9817
Annelien MorlionOncoRNALab, Cancer Research Institute Ghent (CRIG), Ghent University, 9000 Ghent, Belgium.ORCID 0000-0003-1791-2359
Kimberly VerniersOncoRNALab, Cancer Research Institute Ghent (CRIG), Ghent University, 9000 Ghent, Belgium.
Jasper AnckaertOncoRNALab, Cancer Research Institute Ghent (CRIG), Ghent University, 9000 Ghent, Belgium.ORCID 0000-0001-8144-4639
Jan TavernierDepartment of Biomolecular Medicine, Ghent University, 9000 Ghent, Belgium.
Fritz OffnerDepartment of Hematology, Ghent University Hospital, 9000 Ghent, Belgium.
Jo VandesompeleOncoRNALab, Cancer Research Institute Ghent (CRIG), Ghent University, 9000 Ghent, Belgium.ORCID 0000-0001-6274-0184

Funding

Baekeland mandate HBC.2021.0231Ghent University BOF-GOA REINFORCEResearch Foundation - Flanders 11C1623NResearch Foundation - Flanders 11H7523NResearch Foundation - Flanders G0B2820N
6 · The paper itself

Abstract

The potential of RNA-based liquid biopsy is increasingly being recognized in diffuse large B-cell lymphoma (DLBCL), the most common subtype of non-Hodgkin's lymphoma. This study explores the cell-free transcriptome in a humanized DLBCL patient-derived tumor xenograft (PDTX) model. Blood plasma samples (n = 171) derived from a DLBCL PDTX model, including 27 humanized (HIS) PDTX, 8 HIS non-PDTX, and 21 non-HIS PDTX non-obese diabetic (NOD)-scid IL2Rgnull (NSG) mice were collected during humanization, xenografting, treatment, and sacrifice. The mice were treated with either rituximab, cyclophosphamide, doxorubicin, vincristine, and prednisone (R-CHOP), CD20-targeted human IFNα2-based AcTaferon combined with CHOP (huCD20-Fc-AFN-CHOP), or phosphate-buffered saline (PBS). RNA was extracted using the miRNeasy serum/plasma kit and sequenced on the NovaSeq 6000 platform. RNA sequencing data of the formalin-fixed paraffin-embedded (FFPE) tissue and blood plasma samples of the original patient were included. Flow cytometry was performed on immune cells isolated from whole blood, spleen, and bone marrow. Bulk deconvolution was performed using the Tabula Sapiens v1 basis matrix. Both R-CHOP and huCD20-Fc-AFN-CHOP were able to control tumor growth in most mice. Xenograft tumor volume was strongly associated with circulating tumor RNA (ctRNA) concentration (

Indexed as

Antineoplastic Combined Chemotherapy ProtocolsCyclophosphamideLymphoma, Large B-Cell, DiffuseMice, Inbred NODMice, SCIDPrednisoneRituximabTranscriptomeVincristineXenograft Model Antitumor AssaysAnimalsBiomarkers, TumorCell-Free Nucleic AcidsDoxorubicinFemaleGene Expression Regulation, NeoplasticBiomarkers, TumorCell-Free Nucleic AcidsCyclophosphamideDoxorubicinPrednisoneR-CHOP protocolRituximabVincristinebiomarkerscell-free RNAdiffuse large B-cell lymphomaDLBCLliquid biopsypatient-derived tumor xenograft modelPDTX

Identifiers

PMID39337470
PMCPMC11432451

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.