Evidence map›Paper›PMID 39337258›Full record

ArticleInternational journal of molecular sciences2024

A New Real-Time Simple Method to Measure the Endogenous Nitrate Reductase Activity (Nar) in

José J García-Trejo, Sharon Rojas-Alcantar, Monserrat Alonso-Vargas, Raquel Ortega, Alejandro Benítez-Guzmán, Leticia Ramírez-Silva, Natalia Pavón, Claudia Peña-Segura, Ofelia Méndez-Romero, Salvador Uribe-Carvajal and 1 more

Abstract read
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In one paragraph

Article in International journal of molecular sciences, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

José J García-TrejoDepartamento de Biología, Facultad de Química, Universidad Nacional Autónoma de México (U.N.A.M.), Ciudad de México 04510, Mexico.ORCID 0000-0003-2597-9457
Sharon Rojas-AlcantarDepartamento de Biología, Facultad de Química, Universidad Nacional Autónoma de México (U.N.A.M.), Ciudad de México 04510, Mexico.
Monserrat Alonso-VargasLaboratorio de Bioprocesos Ambientales, Universidad Politécnica de Pachuca (U.P.P.), Zempoala, Pachuca 43830, Mexico.ORCID 0000-0002-4103-3162
Raquel OrtegaDepartamento de Biología, Facultad de Química, Universidad Nacional Autónoma de México (U.N.A.M.), Ciudad de México 04510, Mexico.
Alejandro Benítez-GuzmánDepartamento de Microbiología e Inmunología, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México (U.N.A.M.), Ciudad de México 04510, Mexico.
Leticia Ramírez-SilvaDepartamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México (U.N.A.M.), Ciudad de México 04510, Mexico.ORCID 0000-0002-0488-6385
Natalia PavónDepartamento de Farmacología, Instituto Nacional de Cardiología "Ignacio Chávez", Ciudad de México 14080, Mexico.
Claudia Peña-SeguraDepartamento de Bioterio, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México (U.N.A.M.), Ciudad de México 04510, Mexico.
Ofelia Méndez-RomeroDepartamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México (U.N.A.M.), Ciudad de México 04510, Mexico.
Salvador Uribe-CarvajalDepartamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México (U.N.A.M.), Ciudad de México 04510, Mexico.
Arturo Cadena-RamírezLaboratorio de Bioprocesos Ambientales, Universidad Politécnica de Pachuca (U.P.P.), Zempoala, Pachuca 43830, Mexico.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The transmembrane nitrate reductase (Nar) is the first enzyme in the dissimilatory alternate anaerobic nitrate respiratory chain in denitrifying bacteria. To date, there has been no real-time method to determine its specific activity embedded in its native membrane; here, we describe such a new method, which is useful with the inside-out membranes of

Indexed as

Computer SystemsDenitrificationEnzyme AssaysNitrate ReductaseParacoccus denitrificansAnaerobiosisElectron Transport Complex IKineticsNADH DehydrogenaseNitratesNitrogenOxygenTime FactorsElectron Transport Complex INADH DehydrogenaseNitrate ReductaseNitratesNitrogenOxygensodium nitrideactivityBrucelladenitrifiersenteropathogenicmethodNarnewnitrate reductaseParacoccus denitrificansreal-time

Identifiers

PMID39337258
PMCPMC11431489

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.