Evidence map›Paper›PMID 39334525›Full record

ArticleAdvanced biology2025

Spatiotemporal Control Over Protein Release from Artificial Cells via a Light-Activatable Protease.

Arjan Hazegh Nikroo, Wiggert J Altenburg, Thijs W van Veldhuisen, Luc Brunsveld, Jan C M van Hest

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Review
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Arjan Hazegh NikrooLaboratory of Bio-Organic Chemistry, Department of Biomedical Engineering, and Institute for Complex Molecular Systems, Eindhoven University of Technology, P.O. Box 513, Eindhoven, 5600 MB, The Netherlands.ORCID https://orcid.org/0009-0003-1774-3409
Wiggert J AltenburgLaboratory of Bio-Organic Chemistry, Department of Biomedical Engineering, and Institute for Complex Molecular Systems, Eindhoven University of Technology, P.O. Box 513, Eindhoven, 5600 MB, The Netherlands.
Thijs W van VeldhuisenLaboratory of Bio-Organic Chemistry, Department of Biomedical Engineering, and Institute for Complex Molecular Systems, Eindhoven University of Technology, P.O. Box 513, Eindhoven, 5600 MB, The Netherlands.
Luc BrunsveldLaboratory of Chemical Biology, Department of Biomedical Engineering, and Institute for Complex Molecular Systems, Eindhoven University of Technology, P.O. Box 513, Eindhoven, 5600 MB, The Netherlands.
Jan C M van HestLaboratory of Bio-Organic Chemistry, Department of Biomedical Engineering, and Institute for Complex Molecular Systems, Eindhoven University of Technology, P.O. Box 513, Eindhoven, 5600 MB, The Netherlands.ORCID https://orcid.org/0000-0001-7973-2404

Funding

Ministerie van Onderwijs, Cultuur en Wetenschap Spinoza premium SPI 71-259Ministerie van Onderwijs, Cultuur en Wetenschap : Gravitation Program 024.001.035Ministerie van Onderwijs, Cultuur en Wetenschap : Gravitation Program 024.005.020
6 · The paper itself

Abstract

The regulation of protein uptake and secretion by cells is paramount for intercellular signaling and complex multicellular behavior. Mimicking protein-mediated communication in artificial cells holds great promise to elucidate the underlying working principles, but remains challenging without the stimulus-responsive regulatory machinery of living cells. Therefore, systems to precisely control when and where protein release occurs should be incorporated in artificial cells. Here, a light-activatable TEV protease (LaTEV) is presented that enables spatiotemporal control over protein release from a coacervate-based artificial cell platform. Due to the presence of Ni

Indexed as

Artificial CellsEndopeptidasesLightEndopeptidasesTEV proteaseartificial cellscoacervatesliquid‐liquid phase separationphotoswitchable proteinsscaffold proteinsTEV protease

Identifiers

PMID39334525
PMCPMC12078871

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.