Evidence map›Paper›PMID 39333328›Full record

ArticleActa parasitologica2024

Seroprevalence of Schistosoma japonicum Infection Among Dogs and Water Buffaloes Using Recombinant Antigen ELISA in New Corella, Davao del Norte, Philippines.

Jose Ma M Angeles, Joseph Romeo O Paner, Elena A Villacorte, Pilarita T Rivera, Shin-Ichiro Kawazu

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Article in Acta parasitologica, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Jose Ma M AngelesDepartment of Parasitology, College of Public Health, University of the Philippines Manila, Manila, Philippines. jmangeles3@up.edu.ph.
Joseph Romeo O PanerCollege of Medicine, University of the Philippines Manila, Manila, Philippines.
Elena A VillacorteDepartment of Parasitology, College of Public Health, University of the Philippines Manila, Manila, Philippines.
Pilarita T RiveraDepartment of Parasitology, College of Public Health, University of the Philippines Manila, Manila, Philippines.
Shin-Ichiro KawazuNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan.

Funding

Grant-in-Aid for Scientific Research 24405044Japan Society for the Promotion of Science P14088Ministry of Education, Culture, Sports, Science and Technology Global COE ProgramMinistry of Health, Labour and Welfare H23-Shinkosaiko-014
6 · The paper itself

Abstract

purposeAnimal reservoirs are important targets for controlling and eliminating schistosomiasis. Prevalence studies showed that dogs (Canis familiaris) and water buffaloes (Bubalus bubalis) are important reservoirs of S. japonicum. Previous studies have validated the use of the recombinant proteins Sj1TR, Sj7TR, and SjTPx-1 in ELISA as diagnostics for dogs and water buffaloes from schistosomiasis-endemic areas. In this study, we aimed to determine the seroprevalence of S. japonicum among dogs and water buffaloes in New Corella, Davao del Norte, Philippines, using the recombinant proteins Sj1TR, Sj7TR, and SjTPx-1 in ELISA format.

methodsFecal and serum samples were collected from dogs (n = 63) and water buffaloes (n = 57). Schistosome-positive samples were detected using formalin-ether concentration technique (FECT), stool polymerase chain reaction, and enzyme-linked immunosorbent assay (ELISA) using soluble egg antigen (SEA), rSj1TR, rSj7TR, and rSjTPx-1. Positivity rates, sensitivity, specificity, predictive values, accuracy, and kappa values were calculated.

resultsMean positivity rates for schistosome infection were high for both dogs (x = 15.40%) and water buffaloes (x = 6.32%). On dogs, the sensitivity and specificity of the tests are as follows: 66.67% and 100% for rSj7TR-ELISA, while 100% and 93.33% for rSjTPx-1-ELISA, respectively. rSjTPx-1-ELISA showed the highest agreement with stool PCR among all diagnostic tests, with an overall kappa value of 0.824. On water buffaloes, the sensitivity and specificity of both rSj1TR-ELISA and rSjTPx-1-ELISA are 100.0% and 98.15%. Both tests also had an overall kappa value of 0.84.

conclusionTo ensure elimination and prevention of schistosomiasis in humans, the use of validated serological diagnostics such as recombinant antigen ELISA is preferable for field detection in animals, especially in resource-limited areas.

Indexed as

Antigens, HelminthBuffaloesDog DiseasesEnzyme-Linked Immunosorbent AssayFecesSchistosoma japonicumSchistosomiasis japonicaSensitivity and SpecificityAnimalsAntibodies, HelminthDogsPhilippinesRecombinant ProteinsSeroepidemiologic StudiesAntibodies, HelminthAntigens, HelminthRecombinant ProteinsDogsELISARecombinant antigensSchistosoma japonicumWater buffaloes

Identifiers

PMID39333328

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.