Evidence map›Paper›PMID 39332405›Full record

ArticleStem cell reports2024

Functionally redundant roles of ID family proteins in spermatogonial stem cells.

Hue M La, Ai-Leen Chan, Ashlee M Hutchinson, Bianka Y M Su, Fernando J Rossello, Ralf B Schittenhelm, Robin M Hobbs

Abstract read
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In one paragraph

Article in Stem cell reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Hue M LaCentre for Reproductive Health, Hudson Institute of Medical Research, Melbourne, VIC 3168, Australia; Department of Molecular and Translational Sciences, Monash University, Melbourne, VIC 3800, Australia; University of Melbourne Centre for Cancer Research, University of Melbourne, Melbourne, VIC 3000, Australia; Department of Clinical Pathology, University of Melbourne, Melbourne, VIC 3000, Australia.
Ai-Leen ChanCentre for Reproductive Health, Hudson Institute of Medical Research, Melbourne, VIC 3168, Australia; Department of Molecular and Translational Sciences, Monash University, Melbourne, VIC 3800, Australia.
Ashlee M HutchinsonCentre for Reproductive Health, Hudson Institute of Medical Research, Melbourne, VIC 3168, Australia; Department of Molecular and Translational Sciences, Monash University, Melbourne, VIC 3800, Australia.
Bianka Y M SuCentre for Reproductive Health, Hudson Institute of Medical Research, Melbourne, VIC 3168, Australia; Department of Molecular and Translational Sciences, Monash University, Melbourne, VIC 3800, Australia.
Fernando J RosselloDepartment of Clinical Pathology, University of Melbourne, Melbourne, VIC 3000, Australia; Murdoch Children's Research Institute, The Royal Children's Hospital, Melbourne, VIC 3052, Australia; Novo Nordisk Foundation Center for Stem Cell Medicine, Murdoch Children's Research Institute, Melbourne, VIC 3052, Australia; Australian Regenerative Medicine Institute, Monash University, Melbourne, VIC 3800, Australia.
Ralf B SchittenhelmMonash Proteomics & Metabolomics Platform, Monash Biomedicine Discovery Institute & Department of Biochemistry and Molecular Biology, Monash University, Clayton, VIC 3800, Australia.
Robin M HobbsCentre for Reproductive Health, Hudson Institute of Medical Research, Melbourne, VIC 3168, Australia; Department of Molecular and Translational Sciences, Monash University, Melbourne, VIC 3800, Australia. Electronic address: robin.hobbs@monash.edu.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Spermatogonial stem cells (SSCs) are essential for sustained sperm production, but SSC regulatory mechanisms and markers remain poorly defined. Studies have suggested that the Id family transcriptional regulator Id4 is expressed in SSCs and involved in SSC maintenance. Here, we used reporter and knockout models to define the expression and function of Id4 in the adult male germline. Within the spermatogonial pool, Id4 reporter expression and inhibitor of DNA-binding 4 (ID4) protein are found throughout the GFRα1+ fraction, comprising the self-renewing population. However, Id4 deletion is tolerated by adult SSCs while revealing roles in meiotic spermatocytes. Cultures of undifferentiated spermatogonia could be established following Id4 deletion. Importantly, ID4 loss in undifferentiated spermatogonia triggers ID3 upregulation, and both ID proteins associate with transcription factor partner TCF3 in wild-type cells. Combined inhibition of IDs in cultured spermatogonia disrupts the stem cell state and blocks proliferation. Our data therefore demonstrate critical but functionally redundant roles of IDs in SSC function.

Indexed as

Glial Cell Line-Derived Neurotrophic Factor ReceptorsInhibitor of Differentiation ProteinsSpermatogoniaAdult Germline Stem CellsAnimalsBasic Helix-Loop-Helix ProteinsCell DifferentiationCell ProliferationCells, CulturedMaleMiceMice, KnockoutSpermatocytesSpermatogenesisStem CellsTranscription Factor 3Basic Helix-Loop-Helix ProteinsGfra1 protein, mouseGlial Cell Line-Derived Neurotrophic Factor ReceptorsIdb3 protein, mouseIdb4 protein, mouseInhibitor of Differentiation ProteinsTcf3 protein, mouseTranscription Factor 3germlineID genesspermatogonial stem cellstranscription factors

Identifiers

PMID39332405
PMCPMC11561458

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.