Evidence map›Paper›PMID 39331961›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2024

Induced Pluripotent Stem Cell-Derived Parathyroid Organoids Resemble Parathyroid Morphology and Function.

Selinay Şenkal-Turhan, Ezgi Bulut-Okumuş, Muhterem Aydın, Neşe Başak Türkmen, Aslı Taşlıdere, Fikrettin Şahin, Şahin Yılmaz, Pınar Akkuş Süt, Ayşegül Doğan

Abstract read
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In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Article
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  3. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Selinay Şenkal-TurhanDepartment of Genetics and Bioengineering, Faculty of Engineering, Yeditepe University, İstanbul, 34755, Turkey.
Ezgi Bulut-OkumuşDepartment of Genetics and Bioengineering, Faculty of Engineering, Yeditepe University, İstanbul, 34755, Turkey.
Muhterem AydınDepartment of Veterinary Obstetrics and Gynecology, Faculty of Veterinary Medicine, University of Fırat, Elazığ, 23119, Turkey.
Neşe Başak TürkmenDepartment of Pharmaceutical Toxicology, Faculty of Pharmacy, University of Inonu, Malatya, 44280, Turkey.
Aslı TaşlıdereDepartment of Histology and Embryology, Faculty of Medicine, University of Inonu, Malatya, 44280, Turkey.
Fikrettin ŞahinDepartment of Genetics and Bioengineering, Faculty of Engineering, Yeditepe University, İstanbul, 34755, Turkey.
Şahin YılmazDepartment of Genetics and Bioengineering, Faculty of Engineering, Yeditepe University, İstanbul, 34755, Turkey.
Pınar Akkuş SütDepartment of Genetics and Bioengineering, Faculty of Engineering, Yeditepe University, İstanbul, 34755, Turkey.
Ayşegül DoğanDepartment of Genetics and Bioengineering, Faculty of Engineering, Yeditepe University, İstanbul, 34755, Turkey.ORCID 0000-0003-4160-2270

Funding

Scientific and Technological Research Council of Turkey (TUBITAK) 219S665Turkish Academy of Sciences (Outstanding Young Scientists Award) TÜBA-GEBİP 2020
6 · The paper itself

Abstract

The primary role of the parathyroid glands is to maintain calcium homeostasis through the secretion of parathyroid hormone (PTH). The limited proliferative capacity and differentiation of parathyroid cells hinder the generation of cell therapy options. In this study, parathyroid organoids are successfully generated from human-induced pluripotent stem cells (hiPSCs). At the end of the 20 days of differentiation, the parathyroid organoids exhibited distinct parathyroid morphology. Stereomicroscope, scanning electron microscopy (SEM), and transmission electron microscopy (TEM) analysis demonstrated the 3D arrangement of the cell layers in which intracellular structures of parathyroid cells resemble human parathyroid cellular morphology. Comprehensive molecular analyses, including RNA sequencing (RNA-Seq) and liquid chromatography/mass spectrometry (LC-MS/MS), confirmed the expression of key parathyroid-related markers. Protein expression of CasR, CxCr4, Gcm2, and PTH are observed in parathyroid organoids. Parathyroid organoids secrete PTH, demonstrate active intercellular calcium signaling, and induce osteogenic differentiation via their secretome. The tissue integration potential of parathyroid organoids is determined by transplantation into parathyroidectomized rats. The organoid transplanted animals showed significant elevations in PTH-related markers (CasR, CxCr4, Foxn1, Gcm2, and PTH). PTH secretion is detected in organoid-transplanted animals. The findings represent a significant advancement in parathyroid organoid culture and may offer a cellular therapy for treating PTH-related diseases, including hypoparathyroidism.

Indexed as

Cell DifferentiationInduced Pluripotent Stem CellsOrganoidsParathyroid GlandsAnimalsHumansParathyroid HormoneRatsParathyroid Hormone3D culturehypoparathyroidismorganoidparathyroidparathyroid organoidpluripotent stem cell

Identifiers

PMID39331961
PMCPMC11578294

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.