ArticleAndrology2025
Ascorbic acid 2-glucoside improves survival, quality, and fertility of frozen-thawed C57Bl/6J and C57Bl/6N mouse spermatozoa.
Article in Andrology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
4 citing papers in PubMed.
- Ascorbic acid mitigates oxidative structural degradation in bovine spermatozoa: a label-free quantitative phase microscopy study.Veterinary and animal science · 2026Article
- Improvement of Mouse Spermatozoa Freezing at -80°C With Ascorbic Acid 2-Glucoside at Thawing Phase.Andrology · 2026Article
- Essential Oil Blend and Ascorbic Acid Supplementation Improves Performance, Semen Characteristics, Redox Balance, and Intestinal Integrity in Heat-Stressed Male Rabbits.Veterinary sciences · 2026Article
- Ascorbic acid 2-glucoside improves survival, quality, and fertility of frozen-thawed C57Bl/6J and C57Bl/6N mouse spermatozoa.Andrology · 2025Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
backgroundAscorbic acid 2-glucoside (AA2G) is a stabilized form of ascorbic acid and a potent antioxidant. Ascorbic acid is present in the testes and epididymis and helps maintain the physiological integrity of reproductive organs. Its properties have been utilized to protect spermatozoa of different species from oxidative stress. MATERIALS AND
methodsSpermatozoa of C57Bl/6J and C57Bl/6N strains were frozen and analyzed, after thawing, by supplementing the capacitation medium with AA2G, both in the presence and absence of methyl-β-cyclodextrin (MBCD). The effect of treatment was evaluated by SCA System (Microptic) analyzing the velocity, vitality, morphology, and the DNA fragmentation. We also examined sperm capacitation (CTC), acrosome reaction (Coomassie Brillant Blue), and fertility (in vitro fertilization) of treated spermatozoa.
resultsAA2G improved sperm quality and fertility particularly in association with MBCD. We observed a significant increase of sperm motility, velocity, and vitality associated with an enhanced capacitation and acrosome reaction. These improvements resulted in a marked increase in in vitro fertilization success. Embryos obtained were cultured and reached normally the blastocyst stage. DISCUSSION: This study aimed to determine if AA2G could safeguard mouse spermatozoa during cryopreservation. We found a protective effect of AA2G that increased sperm survivability resulting in higher fertilization rate.
conclusionThis newly improved protocol shows potential for reanimating cryopreserved GEMMs stored in mouse biobanks and international repositories, such as the European Mouse Mutant Archive (EMMA). This can serve as a pivotal tool in fulfilling the 3Rs mission (replacement, reduction, and refinement), promoting ethical and humane research practices.
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