Evidence map›Paper›PMID 39330598›Full record

ArticleToxics2024

Evaluation of Spontaneous Overtime Methemoglobin Formation in Post-Mortem Blood Samples from Real Cases in Critical Storage Conditions.

Sara Gariglio, Maria Chiara David, Alessandro Mattia, Francesca Consalvo, Matteo Scopetti, Martina Padovano, Stefano D'Errico, Donato Morena, Paola Frati, Alessandro Santurro and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Toxics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Sara GariglioDIFAR-Department of Pharmacy, University of Genova, 16132 Genova, Italy.ORCID 0000-0002-9507-8155
Maria Chiara DavidDepartment of Public Security, Health Central Directorate, Research Center and Forensic Toxicology Laboratory, Ministry of the Interior, 00184 Rome, Italy.ORCID 0000-0003-0976-5504
Alessandro MattiaDepartment of Public Security, Health Central Directorate, Research Center and Forensic Toxicology Laboratory, Ministry of the Interior, 00184 Rome, Italy.ORCID 0000-0002-9051-4018
Francesca ConsalvoDepartment of Medicine, Surgery and Dentistry, Schola Medica Salernitana, University of Salerno, 84081 Baronissi, Italy.ORCID 0009-0005-8467-249X
Matteo ScopettiDepartment of Medical Surgical Sciences and Translational Medicine, Sapienza University of Rome, 00189 Rome, Italy.ORCID 0000-0002-7259-3090
Martina PadovanoDepartment of Anatomical, Histological, Forensic and Orthopedic Sciences, Sapienza University of Rome, 00161 Rome, Italy.ORCID 0000-0003-2452-7926
Stefano D'ErricoDepartment of Medicine, Surgery, and Health, University of Trieste, 34137 Trieste, Italy.ORCID 0000-0003-3689-0001
Donato MorenaDepartment of Anatomical, Histological, Forensic and Orthopedic Sciences, Sapienza University of Rome, 00161 Rome, Italy.ORCID 0000-0001-6473-7632
Paola FratiDepartment of Anatomical, Histological, Forensic and Orthopedic Sciences, Sapienza University of Rome, 00161 Rome, Italy.ORCID 0000-0002-0358-7965
Alessandro SanturroDepartment of Medicine, Surgery and Dentistry, Schola Medica Salernitana, University of Salerno, 84081 Baronissi, Italy.ORCID 0000-0001-5491-3719
Vittorio FineschiDepartment of Anatomical, Histological, Forensic and Orthopedic Sciences, Sapienza University of Rome, 00161 Rome, Italy.ORCID 0000-0002-1686-3236

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Nitrite/nitrate poisoning is an emerging problem, with an ongoing escalation of reported self-administration with suicidal intent in several countries. Nitrites toxicity mainly consists of their interaction with hemoglobin (Hb), causing its oxidization to methemoglobin (MetHb). In order to give support to the correct procedures for the analysis of these cases, this study aims to evaluate spontaneous sample degradation and consequent MetHb formation in the typical storage conditions of a forensic toxicology laboratory. Two different types of samples have been used in this study: the first stage of our study consisted of a retrospective analysis of blood samples obtained by judicial autopsies already stored in the toxicology laboratory, collected over four years (2018-2021), while the samples used for the second stage were appositely collected during judicial autopsies. The data obtained by the application of a derivative spectrophotometry method on these samples suggest that there seems not to be a maximum threshold for MetHb formation within which it is possible to state with a sufficient grade of certainty that the concentration of MetHb found is consistent with an ante-mortem formation and is not the result of an artifact due to sample degradation and storage conditions. On the other hand, the results suggest that MetHb formation depends on the time passed between sample collection and analysis, so that a tempestive sample processing, performed as soon as the samples are received in the laboratory, is crucial to obtain the maximum reliability and diagnostic values from the data when MetHb quantitation is necessary.

Indexed as

forensic toxicologymethemoglobinnitratesnitritespost-mortem investigationsample degradationspectrophotometrystoragesuicide

Identifiers

PMID39330598
PMCPMC11435480

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.