Evidence map›Paper›PMID 39329754›Full record

ArticleCells2024

RNA-Seq Analysis Revealed circRNAs Associated with Resveratrol-Induced Apoptosis of Porcine Ovarian Granulosa Cells.

Huibin Zhang, Haibo Ye, Hanyu Zhou, Yangguang Liu, Fan Xie, Qianqian Wang, Zongjun Yin, Xiaodong Zhang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cells, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. Article
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  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Huibin ZhangCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.ORCID 0000-0002-4626-1236
Haibo YeCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.ORCID 0009-0004-8599-2260
Hanyu ZhouCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
Yangguang LiuCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
Fan XieCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
Qianqian WangCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.
Zongjun YinCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.ORCID 0000-0001-9893-743X
Xiaodong ZhangCollege of Animal Science and Technology, Anhui Agricultural University, Hefei 230036, China.

Funding

National Key Research and Development Program of China 2021YFD1301200National Natural Science Foundation of China 31972531the Cooperative Innovation Project of Anhui provincial universities GXXT-2021-055the Joint Research Project on Local Pig Breeding in Anhui Province 340000211260001000431the Major Special Science and Technology Project of Anhui Province 202103a06020013
6 · The paper itself

Abstract

Circular RNAs (circRNAs) are a class of circular non-coding RNAs that play essential roles in the intricate and dynamic networks governing cell growth, development, and apoptosis. Resveratrol (RSV), a non-flavonoid polyphenol, is known to participate in follicular development and ovulation. In our previous research, we established a model using porcine ovarian granulosa cells (POGCs) treated with resveratrol, which confirmed its regulatory effects on long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) within these cells. However, the influence of resveratrol on circRNA expression has not been thoroughly investigated. To explore how resveratrol affects circRNA levels in POGCs, we designed an experiment with three groups: a control group (CON, n = 3, 0 μM RSV), a low-dose RSV group (LOW, n = 3, 50 μM RSV), and a high-dose RSV group (HIGH, n = 3, 100 μM RSV) for circRNA sequencing. We identified a total of 10,045 candidate circRNAs from POGCs treated with different concentrations of resveratrol (0, 50, and 100 μM). Differential expression analysis indicated that 96 circRNAs were significantly altered in the LOW vs. CON group, while 109 circRNAs showed significant changes in the HIGH vs. CON group. These circRNAs were notably enriched in biological processes associated with cell metabolism, apoptosis, and oxidative stress. Functional enrichment analysis of the host genes revealed their involvement in critical signaling pathways, including mTOR, AMPK, and apoptosis pathways. Additionally, we identified potential miRNA sponge candidates among the differentially expressed circRNAs, particularly novel_circ_0012954 and novel_circ_0004762, which exhibited strong connectivity within miRNA-target networks. Our findings provide valuable insights into the regulatory mechanisms of circRNAs in the context of resveratrol-induced apoptosis in POGCs, highlighting their potential as innovative therapeutic targets in reproductive biology.

Indexed as

ApoptosisGranulosa CellsResveratrolRNA, CircularRNA-SeqAnimalsFemaleGene Expression RegulationMicroRNAsSwineMicroRNAsResveratrolRNA, CircularapoptosiscircRNAovaryporcineresveratrol

Identifiers

PMID39329754
PMCPMC11429535

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.