Evidence map›Paper›PMID 39324702›Full record

ArticleCancer reports (Hoboken, N.J.)2024

Pseudonormal Morphology of Salivary Gland Adenoid Cystic Carcinoma Cells Subverts the Antitumor Reactivity of Immune Cells: A Tumour-Cell-Based Initiation of Immune Evasion.

Rajdeep Chakraborty, Thiri Zaw, Pallavi Khodlan, Charbel Darido, Giuseppe Palmisano, Arthur Chien, Aidan Tay, Shoba Ranganathan, Fei Liu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancer reports (Hoboken, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Rajdeep ChakrabortyApplied Biosciences, Faculty of Science and Engineering, Macquarie University, Sydney, New South Wales, Australia.ORCID 0000-0001-8210-0459
Thiri ZawAustralian Proteome Analysis Facility, Faculty of Science and Engineering, Macquarie University, Sydney, New South Wales, Australia.
Pallavi KhodlanApplied Biosciences, Faculty of Science and Engineering, Macquarie University, Sydney, New South Wales, Australia.
Charbel DaridoPeter MacCallum Cancer Centre, Melbourne, Victoria, Australia.
Giuseppe PalmisanoSchool of Natural Sciences, Faculty of Science and Engineering, Macquarie University, Sydney, New South Wales, Australia.
Arthur ChienSchool of Natural Sciences, Faculty of Science and Engineering, Macquarie University, Sydney, New South Wales, Australia.
Aidan TayApplied Biosciences, Faculty of Science and Engineering, Macquarie University, Sydney, New South Wales, Australia.
Shoba RanganathanApplied Biosciences, Faculty of Science and Engineering, Macquarie University, Sydney, New South Wales, Australia.
Fei LiuSchool of Natural Sciences, Faculty of Science and Engineering, Macquarie University, Sydney, New South Wales, Australia.

Funding

Australian and New Zealand Head and Neck Cancer Society
6 · The paper itself

Abstract

introductionSalivary gland adenoid cystic carcinoma (ACC), mucoepidermoid carcinoma (MEC) and oral squamous cell carcinoma (OSCC) occurs within the head and neck region. So far immune check point inhibitors failed in ACC. Gipie (CCDC88B) is a microtubule linker protein that activates immune cells. Gipie expressions found in head and neck cancer cells. We hypothesised that the presence of Gipie diminishes anti-tumour reactivity of immune cells towards head and neck cancer.

methodTo determine the effect of Gipie in oral and salivary gland cancer cells, Gipie was silenced in cancer cells in cancer-immune cells co-culture models and we performed 3D Z series confocal imaging, annexin V and immune activation flow cytometry, proteome profiler and discovery phase proteomics.

resultsACC cells morphed into pseudonormal morphology in immune co-culture models. Silencing Gipie in ACC cells showed significant increase of apoptotic cells and activated natural killer cells, and lowering of regulatory T cells. Other salivary and oral cancer cells showed negligible effect of Gipie. Proteome profiler and proteomics assay confirmed Gipie affecting proliferation mechanism and immune activated proteins in ACC immune co-culture models.

conclusionOverall, we conclude that the presence of Gipie has a confounding role during the ACC-immune cell interaction.

Indexed as

Carcinoma, Adenoid CysticCoculture TechniquesSalivary Gland NeoplasmsApoptosisCell Line, TumorHumansKiller Cells, NaturalProteomicsT-Lymphocytes, RegulatoryTumor Escapeadenoid cystic carcinomaanti‐tumour reactivityapoptosiscancerGipiehead and neck cancerimmunotherapymucoepidermoid carcinomaoral canceroral squamous cell carcinoma

Identifiers

PMID39324702
PMCPMC11425664

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.