ArticleNature chemical biology2025
Direct mapping of tyrosine sulfation states in native peptides by nanopore.
Article in Nature chemical biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Identification and Discrimination of Sulfated and Unsulfated Gut-Hormone Biomarkers in Human Serum by Nanopore.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- A self-assembled peptide forms α-helical nanopores for ultrasensitive biomarker profiling.Nature nanotechnology · 2026Article
- Sub-Nanomolar Detection and Discrimination of Microcystin Congeners Using Aerolysin Nanopores.ACS nano · 2026Article
- Exopeptidase-assisted nanopore peptide sequence identification.Nature communications · 2026Article
- Site-specific post-translational modification detection by polar charged engineered MspA nanopores.Chemical science · 2026Article
- Epx4 Nanopore With Multiple Constrictions for Single-Molecule Identification.Small methods · 2026Article
- Nanopore Fingerprinting of Neurodegenerative Proteins and Phosphoproteins within a Minute.JACS Au · 2026Article
- Nanopipette-DNA-Nanopore Hybrid System for Small Molecule Detection.ChemPlusChem · 2026Article
- Data storage and retrieval with unnatural proteins expressed via E. coli.Nature communications · 2026Article
- A Label-Free Nanopore Biosensor for Rapid and Highly Sensitive Detection of Actinomycin in Human Serum.ACS measurement science au · 2026Article
- Nanopore-Based Detection of Protein Posttranslational Modifications.Methods in molecular biology (Clifton, N.J.) · 2026Article
- De Novo Design of α-helical Peptide Nanopores for Single-Molecule Detection Using Helix Packing Motifs.ACS nano · 2025Article
- Reply to: Mass spectrometry and enzyme assays refute histone tyrosine sulfation.Nature chemical biology · 2025Article
- Unwinding process of DNA/RNA quadruplexes by proteins under label-free nanopore monitoring.Nucleic acids research · 2025Article
- Single-molecule nanopore sensing of prolineChemical science · 2025Article
- Regulation of Protein Transport in Functionalized PET Nanopores.The journal of physical chemistry. B · 2025Article
- Transmembrane voltage-gated nanopores controlled by electrically tunable in-pore chemistry.Nature communications · 2025Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Sulfation is considered the most prevalent post-translational modification (PTM) on tyrosine; however, its importance is frequently undervalued due to difficulties in direct and unambiguous determination from phosphorylation. Here we present a sequence-independent strategy to directly map and quantify the tyrosine sulfation states in universal native peptides using an engineered protein nanopore. Molecular dynamics simulations and nanopore mutations reveal specific interactions between tyrosine sulfation and the engineered nanopore, dominating identification across diverse peptide sequences. We show a nanopore framework to discover tyrosine sulfation in unknown peptide fragments digested from a native protein and determine the sequence of the sulfated fragment based on current blockade enhancement induced by sulfation. Moreover, our method allows direct observation of peptide sulfation in ultra-low abundance, down to 1%, and distinguishes it from isobaric phosphorylation. This sequence-independent strategy suggests the potential of nanopore to explore specific PTMs in real-life samples and at the omics level.
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