Evidence map›Paper›PMID 39318513›Full record

ArticleJournal of veterinary research2024

The influence of probiotic administration on the phagocytic and oxidative burst activity of neutrophils and monocytes in the peripheral blood of dairy cows during different lactation periods.

Piotr Brodzki, Hubert Gorzkoś, Jan Marczuk, Urszula Lisiecka, Andrzej Junkuszew, Leszek Krakowski, Marek Szczubiał, Nikodem Brodzki, Katarzyna Głodkowska

Abstract read
In one paragraph

Article in Journal of veterinary research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. The applicability of the MigratestBMC veterinary research · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Piotr BrodzkiDepartment and Clinic of Animal Reproduction, University of Life Sciences in Lublin, 20-612 Lublin, Poland.ORCID https://orcid.org/0000-0002-0809-2101
Hubert GorzkośPrivate Veterinary Practice, Gorvet, 09-227 Sztutowo, Poland.
Jan MarczukDepartment and Clinic of Animal Internal Medicine, University of Life Sciences in Lublin, 20-612 Lublin, Poland.ORCID https://orcid.org/0000-0002-9694-1926
Urszula LisieckaDepartment of Epizootiology and Clinic of Infectious Diseases, University of Life Sciences in Lublin, 20-612 Lublin, Poland.ORCID https://orcid.org/0000-0001-9521-6581
Andrzej JunkuszewDepartment of Breeding of Small Ruminants, Faculty of Biology, Animal Sciences and Bioeconomy, University of Life Sciences in Lublin, 20-028 Lublin, Poland.ORCID https://orcid.org/0000-0003-2679-9597
Leszek KrakowskiDepartment and Clinic of Animal Reproduction, University of Life Sciences in Lublin, 20-612 Lublin, Poland.ORCID https://orcid.org/0000-0001-8868-5670
Marek SzczubiałDepartment and Clinic of Animal Reproduction, University of Life Sciences in Lublin, 20-612 Lublin, Poland.ORCID https://orcid.org/0000-0001-8622-2381
Nikodem BrodzkiFaculty of Veterinary Medicine, University of Life Sciences in Lublin, 20-612 Lublin, Poland.
Katarzyna GłodkowskaDepartment and Clinic of Animal Reproduction, University of Life Sciences in Lublin, 20-612 Lublin, Poland.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: The objective of this bovine peripheral blood study was a comparative assessment of the phagocytic activity of neutrophils and monocytes and of the intracellular killing capacity of neutrophils from cows given no probiotic and from cows which were administered a probiotic consisting of Material and Methods: A cohort of 20 pregnant dairy cows was divided into two groups of 10. The experimental group consisted of cows fed a ration supplemented with probiotics, and the control group consisted of cows fed an unsupplemented ration. Blood was drawn six times: 7 days before dry off, 14 days before parturition, and 7, 21, 60 and 90 days postpartum (DPP). The phagocytic activity of neutrophils and monocytes and the oxidative burst activity of neutrophils were determined by flow cytometry in the peripheral blood of all examined cows. Results: Phagocytosis testing revealed increased percentages of phagocytic neutrophils and monocytes in the experimental group at 21, 60 and 90 DPP (P-value < 0.01). The mean fluorescence intensity (MFI) values for neutrophils and monocytes were higher on all days of the study (P-value < 0.01). In oxidative burst testing, the percentages of detected neutrophils and their MFI were increased in the experimental group on all days (P-value < 0.01). Conclusion: The use of probiotics supported dairy cows' immunity throughout the whole experiment. Probiotic supplementation may limit the occurrence of infectious diseases in these animals.

Indexed as

dairy cowsmonocytesneutrophilsoxidative burstphagocytosis

Identifiers

PMID39318513
PMCPMC11418383

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.