Evidence map›Paper›PMID 39317888›Full record

ArticleReproductive sciences (Thousand Oaks, Calif.)2024

Characterization of Amniotic Fluid Ureaplasma Species from Pregnancies Complicated by Preterm Prelabor Rupture of Membranes.

Antonin Libra, Radka Bolehovska, Rudolf Kukla, Ivana Musilova, Ramkumar Menon, Bo Jacobsson, Marian Kacerovsky

Abstract read
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In one paragraph

Article in Reproductive sciences (Thousand Oaks, Calif.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Antonin LibraBiomedical Research Center, University Hospital Hradec Kralove, Sokolska 581, Hradec Kralove, 500 05, Czech Republic.
Radka BolehovskaInstitute of Clinical Microbiology, University Hospital, Hradec Kralove, Czech Republic.
Rudolf KuklaInstitute of Clinical Microbiology, University Hospital, Hradec Kralove, Czech Republic.
Ivana MusilovaBiomedical Research Center, University Hospital Hradec Kralove, Sokolska 581, Hradec Kralove, 500 05, Czech Republic.
Ramkumar MenonDivision of Basic Science & Translational Research, Department of Obstetrics and Gynecology, Medical Branch, The University of Texas, Galveston, TX, USA.
Bo JacobssonDepartment of Obstetrics and Gynecology, Institute of Clinical Science, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Marian KacerovskyBiomedical Research Center, University Hospital Hradec Kralove, Sokolska 581, Hradec Kralove, 500 05, Czech Republic. kacermar@fnhk.cz.ORCID 0000-0001-9858-7900

Funding

Fakultní nemocnice Hradec Králové a long-term developmental plan
6 · The paper itself

Abstract

The main aim of this study was to determine expanded sequence types (eSTs) of Ureaplasma species (U. spp.). DNA isolated from the amniotic fluid of pregnancies complicated by preterm prelabor rupture of membranes (PPROM) using an expanded multilocus sequence typing scheme. Additionally, the study sought to examine whether phylogenetic subgroups of U. spp. DNA differ with respect to maternal demographic and clinical parameters and selected aspects of short-term neonatal morbidity. This retrospective cohort study was focused on singleton pregnancies complicated by PPROM occurring between the gestational ages of 24+0 and 36+6 weeks, where amniocentesis was conducted to assess the intra-amniotic environment and the presence of U. spp. DNA in the amniotic fluid samples was confirmed. The stored aliquots of U. spp. DNA were used to assess differences in nucleotide sequences in six U. spp. genes (ftsH, rpL22, valS, thrS,ureG, and mba-np1) using the eMLST scheme. The expanded multilocus sequence typing scheme was performed in 73 samples of U. spp. DNA isolated from pregnancies complicated by PPROM. In total, 33 different U. spp. DNA eSTs were revealed, 21 (#20, 233-244, 248-251, 253, 255, 259, and 262) of which were novel. The most frequently identified eST was #41, identified in 18% (13/73) of the aliquots. Based on their genetic relationships, the U. spp. DNA was divided into two clusters and four subgroups [cluster I (U. parvum): A, 43% (n = 31); B, 15% (n = 11); and C, 26% (n = 19); cluster II (U. urealyticum): 1; 16% (n = 12)]. Cluster II had a higher rate of polymicrobial findings than cluster I (58% vs 16%; p = 0.005), while subgroup A had the highest rate of concomitant Mycoplasma hominis in the amniotic fluid samples (66%; p = 0.04). In conclusion, Ureaplasma spp. DNA obtained from PPROM consisted of 33 different eSTs of U. spp. DNA. No differences in maternal and neonatal characteristics were found among the phylogenetical subgroups of U. spp. DNA, except for a higher rate of polymicrobial amniotic fluid findings in those with U. urealyticumand the concomitant presence of M. hominis in the amniotic fluid in those with the presence of U. parvum.

Indexed as

Amniotic FluidMultilocus Sequence TypingPremature Rupture of Fetal MembranesUreaplasmaUreaplasma InfectionsAdultDNA, BacterialFemaleGestational AgeHumansPhylogenyPregnancyPregnancy Complications, InfectiousRetrospective StudiesDNA, BacterialGenital mycoplasmaMicrobial invasion of the amniotic cavityMolecular biologyMollicutesMorbidityNeonatesPreterm deliverySequencing

Identifiers

PMID39317888

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.