Evidence map›Paper›PMID 39316254›Full record

ArticleInternational microbiology : the official journal of the Spanish Society for Microbiology2025

Screening and heterologous expression of an antimicrobial peptide SCAK33 with broad-spectrum antimicrobial activity resourced from sea cucumber proteome.

Yanqiu Zhao, Lujiang Hao, Yiwei Meng, Longfen Li, Weitao Wang, Rui Zhao, Peipei Zhao, Jiyuan Zhang, Mengmeng Wang, Jingli Ren and 3 more

Abstract read
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In one paragraph

Article in International microbiology : the official journal of the Spanish Society for Microbiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Yanqiu Zhao *School of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353, China.
Lujiang Hao *School of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353, China.
Yiwei MengBiology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250103, China.
Longfen LiBiology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250103, China.
Weitao WangBiology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250103, China.
Rui ZhaoBiology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250103, China.
Peipei ZhaoBiology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250103, China.
Jiyuan ZhangBiology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250103, China.
Mengmeng WangBiology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250103, China.
Jingli RenBiology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250103, China.
Lixin ZhangBiology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250103, China.
Xin YinBiology Institute, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250103, China. yinxin@sdas.org.
Xuekui XiaSchool of Bioengineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, 250353, China. xiaxk@sdas.org.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Antimicrobial peptides (AMPs) are a family of short defense proteins that are naturally produced by all organisms and have great potential as effective substitutes for small-molecule antibiotics. The present study aims to excavate AMPs from sea cucumbers and achieve their heterologous expression in prokaryotic Escherichia coli. Using MytC as a probe, a cysteine-stabilized peptide SCAK33 with broad-spectrum antimicrobial activity was discovered from the proteome of Apostichopus japonicas. The SCAK33 showed inhibitory effects on both gram positive and gram negative bacteria with MICs of 3-28 μM, and without significant hemolysis activity in rat blood erythrocyte. Especially, it exhibited good antimicrobial activity against Bacillus megaterium, B. subtilis, and Vibrio parahaemolyticus with the MIC of 3, 7, and 7 μM, respectively. After observation by scanning electronic microscopy (SEM) and confocal laser scanning microscope (CLSM), it was found that the cell membrane of bacteria was severely damaged. Furthermore, the recombinant SCAK33 (reSCAK33) was heterologously expressed by fusion with SUMO tag in E. coli BL21(DE3), and the protein yield reached 70 mg/L. The research will supplement the existing quantity of sea cucumber AMPs and provide data support for rapid mining and biological preparation of sea cucumber AMPs.

Indexed as

Anti-Bacterial AgentsAntimicrobial Cationic PeptidesAntimicrobial PeptidesProteomeSea CucumbersStichopusAnimalsErythrocytesEscherichia coliGene ExpressionGram-Negative BacteriaGram-Positive BacteriaHemolysisMicrobial Sensitivity TestsRatsRecombinant ProteinsAnti-Bacterial AgentsAntimicrobial Cationic PeptidesAntimicrobial PeptidesProteomeRecombinant ProteinsAntimicrobial peptideCLSMHeterologous expressionProteomicSea cucumberSEM

Identifiers

PMID39316254

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.