Evidence map›Paper›PMID 39314058›Full record

ArticleDisease models & mechanisms2024

Comparative transcriptomic analysis of articular cartilage of post-traumatic osteoarthritis models.

Sophie J Gilbert, Jamie Soul, Yao Hao, Hua Lin, Katarzyna A Piróg, Jonathan Coxhead, Krutik Patel, Matt J Barter, David A Young, Emma J Blain

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Disease models & mechanisms, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. LOXL2 alleviates post-traumatic knee osteoarthritis and pain.Journal of orthopaedic translation · 2025
    Article
  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Sophie J GilbertBiomechanics and Bioengineering Research Centre Versus Arthritis, Biomedicine Division, School of Biosciences, The Sir Martin Evans Building, Cardiff University, Cardiff CF10 3AX, Wales, UK.
Jamie SoulBiosciences Institute, Newcastle University, Centre for Life, Central Parkway, Newcastle upon Tyne NE1 3BZ, UK.ORCID 0000-0003-2255-6459
Yao HaoBiosciences Institute, Newcastle University, Centre for Life, Central Parkway, Newcastle upon Tyne NE1 3BZ, UK.
Hua LinBiosciences Institute, Newcastle University, Centre for Life, Central Parkway, Newcastle upon Tyne NE1 3BZ, UK.
Katarzyna A PirógBiosciences Institute, Newcastle University, Centre for Life, Central Parkway, Newcastle upon Tyne NE1 3BZ, UK.
Jonathan CoxheadBiosciences Institute, Newcastle University, Centre for Life, Central Parkway, Newcastle upon Tyne NE1 3BZ, UK.ORCID 0000-0002-6128-9560
Krutik PatelBiosciences Institute, Newcastle University, Centre for Life, Central Parkway, Newcastle upon Tyne NE1 3BZ, UK.
Matt J BarterBiosciences Institute, Newcastle University, Centre for Life, Central Parkway, Newcastle upon Tyne NE1 3BZ, UK.
David A YoungBiosciences Institute, Newcastle University, Centre for Life, Central Parkway, Newcastle upon Tyne NE1 3BZ, UK.ORCID 0000-0002-7078-6745
Emma J BlainBiomechanics and Bioengineering Research Centre Versus Arthritis, Biomedicine Division, School of Biosciences, The Sir Martin Evans Building, Cardiff University, Cardiff CF10 3AX, Wales, UK.ORCID 0000-0001-8944-4254

Funding

Dunhill Medical Trust R476/0516JGW Patterson FoundationMedical Research Council JXR 10641Medical Research Council Canada JXR 10641, MR/P020941/1Newcastle UniversityVersus Arthritis 18461
6 · The paper itself

Abstract

Animal models of post-traumatic osteoarthritis (PTOA) recapitulate the pathological changes observed in human PTOA. Here, skeletally mature C57Bl6 mice were subjected to either rapid-onset non-surgical mechanical rupture of the anterior cruciate ligament (ACL) or to surgical destabilisation of the medial meniscus (DMM). Transcriptome profiling of micro-dissected cartilage at day 7 or day 42 following ACL or DMM procedure, respectively, showed that the two models were comparable and highly correlative. Gene ontology (GO) enrichment analysis identified similarly enriched pathways that were overrepresented by anabolic terms. To address the transcriptome changes more completely in the ACL model, we also performed small RNA sequencing, describing the first microRNA profile of this model. miR-199-5p was amongst the most abundant, yet differentially expressed, microRNAs, and its inhibition in primary human chondrocytes led to a transcriptome response that was comparable to that observed in both human 'OA damaged vs intact cartilage' and murine DMM cartilage datasets. We also experimentally verified CELSR1, GIT1, ECE1 and SOS2 as novel miR-199-5p targets. Together, these data support the use of the ACL rupture model as a non-invasive companion to the DMM model.

Indexed as

Cartilage, ArticularChondrocytesDisease Models, AnimalGene Expression ProfilingMice, Inbred C57BLMicroRNAsOsteoarthritisTranscriptomeAnimalsAnterior Cruciate Ligament InjuriesGene Expression RegulationGene OntologyHumansMaleMiceMicroRNAsMirn199 microRNA, mouseACL ruptureCartilageDMMmicroRNAPTOA

Identifiers

PMID39314058
PMCPMC11524441

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.