Evidence map›Paper›PMID 39313516›Full record

ArticleActa pharmacologica Sinica2025

Adaptor protein Src-homology 2 domain containing E (SH2E) deficiency induces heart defect in zebrafish.

Yu-Lai Liang, Yang-Xi Hu, Fang-Fang Li, Hong-Min You, Jian Chen, Chun Liang, Zhi-Fu Guo, Qing Jing

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta pharmacologica Sinica, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. 1,24,25(OH)Scientific reports · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Yu-Lai Liang *Laboratory of Molecular Immunology, State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, 200438, China.
Yang-Xi Hu *Department of Cardiology, Second Affiliated Hospital of Naval Medical University, Shanghai, 200003, China.
Fang-Fang Li *Department of Cardiology, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200080, China. lifang091001@163.com.
Hong-Min YouDepartment of Cardiovascular Medicine, Changhai Hospital, Naval Medical University, Shanghai, 200433, China.
Jian ChenCAS Key Laboratory of Tissue Microenvironment and Tumor, Innovation Center for Intervention of Chronic Disease and Promotion of Health, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, 200031, China.
Chun LiangDepartment of Cardiology, Second Affiliated Hospital of Naval Medical University, Shanghai, 200003, China.
Zhi-Fu GuoDepartment of Cardiovascular Medicine, Changhai Hospital, Naval Medical University, Shanghai, 200433, China.
Qing JingCAS Key Laboratory of Tissue Microenvironment and Tumor, Innovation Center for Intervention of Chronic Disease and Promotion of Health, Shanghai Institute of Nutrition and Health, University of Chinese Academy of Sciences, Chinese Academy of Sciences, Shanghai, 200031, China. qjing@sibs.ac.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Adaptor proteins play crucial roles in signal transduction across diverse signaling pathways. Src-homology 2 domain-containing E (SH2E) is the adaptor protein highly expressed in vascular endothelial cells and myocardium during zebrafish embryogenesis. In this study we investigated the function and mechanisms of SH2E in cardiogenesis. We first analyzed the spatiotemporal expression of SH2E and then constructed zebrafish lines with SH2E deficiency using the CRISPR-Cas9 system. We showed that homozygous mutants developed progressive pericardial edema (PCE), dilated atrium, abnormal atrioventricular looping and thickened atrioventricular wall from 3 days post fertilization (dpf) until death; inducible overexpression of SH2E was able to partially rescue the PCE phenotype. Using transcriptome sequencing analysis, we demonstrated that the MAPK/ERK and NF-κB signaling pathways might be involved in SH2E-deficiency-caused PCE. This study underscores the pivotal role of SH2E in cardiogenesis, and might help to identify innovative diagnostic techniques and therapeutic strategies for congenital heart disease.

Indexed as

ZebrafishZebrafish ProteinsAdaptor Proteins, Signal TransducingAnimalsCRISPR-Cas SystemsHeart Defects, CongenitalNF-kappa BProto-Oncogene ProteinsSignal TransductionType C PhospholipasesAdaptor Proteins, Signal TransducingNF-kappa Bplcb3 protein, zebrafishProto-Oncogene ProteinsShe protein, mouseType C PhospholipasesZebrafish Proteinscardiac developmentCRISPR/Cas9pericardial edemaSrc-homology 2 domain containing Ezebrafish

Identifiers

PMID39313516
PMCPMC11747093

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.