Evidence map›Paper›PMID 39312036›Full record

ArticleVirus genes2024

Exploring the role of YBX3 in PEDV infection through the utilization of YBX3 knockout and overexpression cell lines.

Jiayun Wu, Huizhen Gao, Haoyu Rui, Pan Xu, Ligang Ni, Junsheng Zhang, Ligang Wang

Abstract read
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In one paragraph

Article in Virus genes, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Jiayun WuJiangsu Agri-Animal Husbandry Vocational College, Taizhou, 22530, China.
Huizhen GaoJiangsu Agri-Animal Husbandry Vocational College, Taizhou, 22530, China.
Haoyu RuiJiangsu Agri-Animal Husbandry Vocational College, Taizhou, 22530, China.
Pan XuJiangsu Agri-Animal Husbandry Vocational College, Taizhou, 22530, China.
Ligang NiJiangsu Agri-Animal Husbandry Vocational College, Taizhou, 22530, China.
Junsheng ZhangJiangsu Agri-Animal Husbandry Vocational College, Taizhou, 22530, China.
Ligang WangJiangsu Agri-Animal Husbandry Vocational College, Taizhou, 22530, China. wlg7909@163.com.

Funding

Natural Science Foundation of Jiangsu Agri-animal Husbandry Vocational College NSF2023CB01Natural Science Research of Jiangsu Higher Education Institutions of China 23KJB230004
6 · The paper itself

Abstract

Porcine epidemic diarrhea (PED) is a highly contagious disease caused by the porcine epidemic diarrhea virus (PEDV), which results in significant economic losses. PEDV infection causes severe damage to the midgut barrier in the small intestine. YBX3, an important protein in tight junctions, promotes epithelial cell proliferation. However, its role in the process of PEDV infection remains unclear. In this study, we observed a significant increase in mRNA expression of YBX3 following PEDV infection. Additionally, the protein expression of YBX3 showed an initial increase followed by a decrease over time. Furthermore, treatment with 2% DSS resulted in a significant down-regulation of YBX3 mRNA and protein expression. Furthermore, we successfully generated knockout and overexpression cell lines of YBX3. Preliminary assays indicated that elevated expression of YBX3 inhibited the PEDV replication, while knockout of YBX3 had the opposite effect. In conclusion, our study has preliminarily revealed the functional role of YBX3 during PEDV infection. This finding lays the foundation for further investigation into its mechanism in future and also provides new insights into the mechanism of PEDV-host interactions.

Indexed as

Porcine epidemic diarrhea virusVirus ReplicationAnimalsCell LineChlorocebus aethiopsCoronavirus InfectionsGene Knockout TechniquesHost-Pathogen InteractionsSwineSwine DiseasesVero CellsPigsPorcine epidemic diarrhea virusTight junctionYBX3

Identifiers

PMID39312036

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.