ArticleThe Journal of antimicrobial chemotherapy2024
Mycobacterium abscessus strain variability in preclinical drug development: does it really matter?
Article in The Journal of antimicrobial chemotherapy, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- The stress response factor SigH mediates intrinsic resistance to multiple antibiotics inMicrobiology spectrum · 2026Article
- Towards enhanced translational value: preclinical drug activity testing against actively multiplying, nutrient-starved and pellicle biofilm-embedded Mycobacterium abscessus.The Journal of antimicrobial chemotherapy · 2026Article
- Construction of a Subcutaneous Fat Transplantation Model Infected with Mycobacterium.Current medicinal chemistry · 2026Article
- In Vitro Activity of Imipenem/Relebactam Alone and in Combination Against Cystic Fibrosis Isolates ofAntibiotics (Basel, Switzerland) · 2025Article
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Authors and funding
5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundNew treatment options for Mycobacterium abscessus infections are urgently needed. Since a correlation between MICs and clinical outcomes is not clearly established, potency of novel drugs needs to be evaluated using additional in vitro drug activity assays. Preclinical drug activity assays generally use the M. abscessus type strain ATCC 19977. However, M. abscessus complex entails a genetically and morphologically diverse group, and it is questionable whether drug activity observed against ATCC 19977 is representative of drug activity against clinical M. abscessus isolates.
objectivesTo assess whether the relationship between MIC and the quantitative antimycobacterial activity of amikacin, imipenem and clofazimine differs between the ATCC 19977 strain and clinical M. abscessus isolates.
methodsExperiments were performed with M. abscessus ATCC 19977 and a subset of six clinical isolates covering the three M. abscessus subspecies and the smooth and rough morphotypes. Cultures were exposed to the drugs at 4-fold increasing, MIC-standardized concentrations, and the mycobacterial load was assessed over time.
resultsConcentration- and time-dependent activity of amikacin, imipenem and clofazimine against the six clinical isolates was similar. Only slight variations in drug activity were observed between ATCC 19977 and clinical isolates.
conclusionsTime- and concentration-dependent drug activity against the ATCC 19977 strain seems indicative for in vitro drug behaviour against M. abscessus complex clinical isolates. Including one clinical smooth morphotype isolate alongside ATCC 19977 seems appropriate for reliable interpretation of this particular in vitro drug activity assay as part of the M. abscessus preclinical drug development pipeline.
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