Comparative Analysis of Silk Fibroin Membranes across Cross-Linking Methods: Processing and Characterization.

Silk fibroin (SF) extracted from silkworm silk can be transformed into transparent membranes with well-suited physical properties for ophthalmic applications. There is ample literature on the fabrication and characterization of SF-based membranes; however, the use of diverse SF extraction protocols and characterization methods or their settings makes it difficult to compare different silk membrane properties across studies. In this work, we fabricated 10 families of SF-based membranes by physical cross-linking and one non-cross-linked as a control. We evaluated transparency (ranging from 84.5 to 95.3% in the visible spectrum), enzyme stability (from 24 h to 200 days in protease XIV), decomposition temperature (280-290 °C), water uptake (40-60%), Young's modulus (8-30 MPa), roughness (1.6-22.7 nm), and FTIR spectra for the secondary structure. We found correlation between water uptake and the Young's modulus (the lower the water uptake, the higher the Young's modulus) and a relationship between membrane stability in protease XIV and the secondary structure of the proteins. Higher surface roughness and faster degradation were found in membranes cross-linked with polyethylene glycol, and conversely, lower roughness and lower degradation were found in methanol, ethanol, or isopropanol crossed-link membranes. This ample compilation of materials and their characterization will aid in the selection of a SF-based material according to the needs of the application.