Evidence map›Paper›PMID 39306657›Full record

ArticleBMC microbiology2024

Whole-genome sequencing of two multidrug-resistant acinetobacter baumannii strains isolated from a neonatal intensive care unit in Egypt: a prospective cross-sectional study.

Rania Alam Eldin Mohamed, Nouran Magdy Moustafa, Fatma Mostafa Mahmoud, Yara Said Elsaadawy, Heba Sherif Abdel Aziz, Shaimaa Abou Bakr Gaber, Abdelrahman Mohamed Hussin, Mohamed G Seadawy

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC microbiology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Tackling carbapenem-resistantBiotechnology notes (Amsterdam, Netherlands) · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Rania Alam Eldin MohamedMedical Microbiology and Immunology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
Nouran Magdy MoustafaMedical Microbiology and Immunology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
Fatma Mostafa MahmoudMedical Microbiology and Immunology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
Yara Said ElsaadawyMedical Microbiology and Immunology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
Heba Sherif Abdel AzizClinical and Chemical Pathology Department, Faculty of Medicine, Cairo University, Cairo, Egypt. dr_heba87@cu.edu.eg.
Shaimaa Abou Bakr GaberClinical Pathology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
Abdelrahman Mohamed HussinArmed Forces College of Medicine, Cairo, Egypt.
Mohamed G SeadawyBiodefense Center for Infectious and Emerging Diseases, Ministry of Defense, Cairo, Egypt.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundAcinetobacter baumannii (A. baumannii) is a life-threatening and challenging pathogen. In addition, it accounts for numerous serious infections, particularly among immunocompromised patients. Resistance to nearly all clinically used antibiotics and their ability to spread this resistance is one of the most important concerns related to this bacterium.

objectivesThis study describes different molecular mechanisms of two multidrug-resistant A. baumannii isolates obtained from endotracheal aspirates collected from the neonatal intensive care unit (NICU), Ain Shams University Hospital, Egypt.

methodsFollowing the identification of two isolates, they were examined for susceptibility to antimicrobial agents. This was followed by multilocus sequence typing as well as whole-genome sequence (WGS). Additionally, a Pathosystems Resources Integration Center (PATRIC) analysis was performed.

resultsTwo isolates, Ab119 and Ab123, exhibited resistance to all tested antibiotics except for tigecycline and colistin. The WGS analysis of antimicrobial resistance genes (AMR) indicated that both isolates shared beta-lactam, aminoglycoside, macrolides, and sulfonamide resistance genes. Furthermore, each strain revealed different resistance genes such as blaNDM-1, blaNDM-10, OXA-64, aph (3')-VI, Tet-B in Ab119 strain and blaOXA-68, blaPER-1, blaPER-7, Tet-39 in Ab123 strain. Multiple efflux pump genes were detected. Multilocus sequence typing indicated that both isolates belong to the same sequence type (ST931), which belongs to international clone (IC3). Both isolates exhibited the presence of multiple mobile genetic elements (MGEs), but no plasmid was detected in either of them.

conclusionsA low prevalence of the IC3 sequence type was identified among two A. baumannii isolates obtained from the NICU in Egypt, exhibiting a high resistance level. Healthcare workers must have knowledge regarding the prevalence of A. baumannii among different populations in order to administer suitable treatment, improve patient outcomes, and apply effective infection control practices.

Indexed as

Acinetobacter baumanniiAcinetobacter InfectionsAnti-Bacterial AgentsDrug Resistance, Multiple, BacterialGenome, BacterialIntensive Care Units, NeonatalMicrobial Sensitivity TestsMultilocus Sequence TypingWhole Genome SequencingCross-Sectional StudiesEgyptHumansInfant, NewbornProspective StudiesAnti-Bacterial AgentsAntibiotic resistance genesMultidrug-resistant a. baumanniiMultilocus sequence typingWhole-genome sequencing

Identifiers

PMID39306657
PMCPMC11415996

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.