Evidence map›Paper›PMID 39306482›Full record

ArticleUltrasound in medicine & biology2024

Ultrasound Induces Similar Temporal Endothelial Expression Patterns of eNOS and KLF2 as Normal Flow.

Jaideep Sahni, Ian S McCue, Adam R Johnson, Morgan A Schake, Luz D Sotelo, Joseph A Turner, Ryan M Pedrigi

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Article in Ultrasound in medicine & biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Jaideep SahniDepartment of Mechanical and Materials Engineering, University of Nebraska-Lincoln, Lincoln, NE, USA.
Ian S McCueDepartment of Mechanical and Materials Engineering, University of Nebraska-Lincoln, Lincoln, NE, USA.
Adam R JohnsonDepartment of Mechanical and Materials Engineering, University of Nebraska-Lincoln, Lincoln, NE, USA.
Morgan A SchakeDepartment of Mechanical and Materials Engineering, University of Nebraska-Lincoln, Lincoln, NE, USA.
Luz D SoteloDepartment of Mechanical and Materials Engineering, University of Nebraska-Lincoln, Lincoln, NE, USA.
Joseph A TurnerDepartment of Mechanical and Materials Engineering, University of Nebraska-Lincoln, Lincoln, NE, USA.
Ryan M PedrigiDepartment of Mechanical and Materials Engineering, University of Nebraska-Lincoln, Lincoln, NE, USA; Nebraska Center for Integrated Biomolecular Communication, University of Nebraska-Lincoln, Lincoln, NE, USA; Department of Cellular and Integrative Physiology, University of Nebraska Medical Center, Omaha, NE, USA. Electronic address: rpedrigi@unl.edu.

Funding

Targeted mass spectrometry approaches to understand CART processing and recepter interactionsP20GM113126 · NIGMS · UNIVERSITY OF NEBRASKA LINCOLN · PI RAZAVI RIZI, MOHAMMAD · 2016 to 2025
$20.8M
ULTRASOUND AS A MECHANOTHERAPY FOR ENDOTHELIAL CELL DYSFUNCTIONR21EB028960 · NIBIB · UNIVERSITY OF NEBRASKA LINCOLN · PI PEDRIGI, RYAN MICHAEL · 2020 to 2020
$603k
NIBIB NIH HHS R21 EB028960NIGMS NIH HHS P20 GM113126
6 · The paper itself

Abstract

objectiveTo determine the sensitivity of vascular endothelial cells to long durations of low-intensity pulsed ultrasound (LIPUS) compared to normal flow and identify the duration that maximizes expression of two mechanosensitive genes related to healthy endothelial function, endothelial nitric oxide synthase (eNOS) and Krüppel-like factor 2 (KLF2).

methodsCustom ultrasound exposure tanks were developed and the acoustic field was characterized. Human umbilical vein endothelial cells were seeded into culture plates and exposed to LIPUS at a frequency of 1 MHz and acoustic pressure of 217 kPa for 20 min, 1 h, 6 h, 9 h, or 24 h. As a comparator, other cells were exposed to normal flow. RT-qPCR was used to assess mRNA expression of eNOS and KLF2.

resultsMaximum eNOS and KLF2 expression occurred at 6 h and was localized to the beam path. Both genes exhibited qualitatively similar patterns of expression under LIPUS compared to normal flow. LIPUS induced a more rapid beneficial response compared to normal flow, but flow induced higher expression of both genes. eNOS expression after 6 h of LIPUS was dependent on RNA yield and culture duration prior to experiments.

conclusionEndothelial cells exposed to longer durations of LIPUS than typically employed exhibited greater expression of beneficial genes. The temporal gene expression patterns resulting from LIPUS and normal flow suggest activation of similar signaling pathways. However, LIPUS also caused increased RNA yield that may be linked to proliferation, which would suggest more of a wound healing than atheroprotective phenotype.

Indexed as

Human Umbilical Vein Endothelial CellsKruppel-Like Transcription FactorsNitric Oxide Synthase Type IIICells, CulturedHumansUltrasonic WavesKLF2 protein, humanKruppel-Like Transcription FactorsNitric Oxide Synthase Type IIINOS3 protein, humanAcoustic pressureAtherosclerosisBiomechanicsEndothelial cellsLow-intensity pulsed ultrasoundShear stressWound healing

Identifiers

PMID39306482
PMCPMC11490374

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