Evidence map›Paper›PMID 39305484›Full record

ArticleCell reports2024

The microtubule regulator EFA-6 forms cortical foci dependent on its intrinsically disordered region and interactions with tubulins.

Anjali Sandhu, Xiaohui Lyu, Xinghaoyun Wan, Xuefeng Meng, Ngang Heok Tang, Gilberto Gonzalez, Ishana N Syed, Lizhen Chen, Yishi Jin, Andrew D Chisholm

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors.

Anjali SandhuDepartment of Neurobiology, School of Biological Sciences, University of California, San Diego, San Diego, CA 92093, USA.
Xiaohui LyuDepartment of Neurobiology, School of Biological Sciences, University of California, San Diego, San Diego, CA 92093, USA.
Xinghaoyun WanDepartment of Neurobiology, School of Biological Sciences, University of California, San Diego, San Diego, CA 92093, USA.
Xuefeng MengDepartment of Neurobiology, School of Biological Sciences, University of California, San Diego, San Diego, CA 92093, USA.
Ngang Heok TangDepartment of Cell and Developmental Biology, School of Biological Sciences, University of California, San Diego, San Diego, CA 92093, USA.
Gilberto GonzalezBarshop Institute for Longevity and Aging Studies, Department of Cell Systems and Anatomy, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229, USA.
Ishana N SyedBarshop Institute for Longevity and Aging Studies, Department of Cell Systems and Anatomy, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229, USA.
Lizhen ChenBarshop Institute for Longevity and Aging Studies, Department of Cell Systems and Anatomy, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229, USA.
Yishi JinDepartment of Neurobiology, School of Biological Sciences, University of California, San Diego, San Diego, CA 92093, USA.
Andrew D ChisholmDepartment of Neurobiology, School of Biological Sciences, University of California, San Diego, San Diego, CA 92093, USA; Department of Cell and Developmental Biology, School of Biological Sciences, University of California, San Diego, San Diego, CA 92093, USA. Electronic address: adchisholm@ucsd.edu.

Funding

Enhancing and expanding the CGC Strain CollectionP40OD010440 · OD · UNIVERSITY OF MINNESOTA · PI Aric L Daul, Ann E. Rougvie · 2012 to 2026
$7.5M
Molecular genetics of axon and synapse development and maintenanceR35NS127314 · NINDS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Yishi Jin · 2022 to 2026
$4.3M
Cytoskeletal dynamics in axon regenerationR01NS093588 · NINDS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI CHISHOLM, ANDREW D, JIN, YISHI · 2015 to 2023
$3.7M
The role of CELF2 and its genetic variants in Alzheimer's diseaseR01AG070214 · NIA · UNIVERSITY OF TEXAS HLTH SCIENCE CENTER · PI CHEN, LIZHEN · 2021 to 2025
$2.2M
Maintenance and Repair of the C. elegans SkinR35GM134970 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI CHISHOLM, ANDREW D · 2020 to 2024
$2.1M
Neuronal microtubule regulation and agingR01AG071591 · NIA · UNIVERSITY OF TEXAS HLTH SCIENCE CENTER · PI Lizhen Chen · 2021 to 2026
$1.6M
Integrated Graduate Training Program in Neuroscience, UTHSCSAT32NS082145 · NINDS · UNIVERSITY OF TEXAS HLTH SCIENCE CENTER · PI David A Morilak · 2013 to 2026
$1.5M
NIA NIH HHS R01 AG070214NIA NIH HHS R01 AG071591NIGMS NIH HHS R35 GM134970NIH HHS P40 OD010440NINDS NIH HHS R01 NS093588NINDS NIH HHS R35 NS127314NINDS NIH HHS T32 NS082145
6 · The paper itself

Abstract

The EFA6 protein family, originally identified as Sec7 guanine nucleotide exchange factors, has also been found to regulate cortical microtubule (MT) dynamics. Here, we find that in the mature C. elegans epidermal epithelium, EFA-6 forms punctate foci in specific regions of the apical cortex, dependent on its intrinsically disordered region (IDR). The EFA-6 IDR can form biomolecular condensates in vitro. In genetic screens for mutants with altered GFP::EFA-6 localization, we identified a gain-of-function (gf) mutation in α-tubulin tba-1 that induces ectopic EFA-6 foci in multiple cell types. Lethality of tba-1(gf) is partially suppressed by loss of function in efa-6. The ability of TBA-1(gf) to trigger ectopic EFA-6 foci requires β-tubulin TBB-2 and the chaperon EVL-20/Arl2. tba-1(gf)-induced EFA-6 foci display slower turnover, contain the MT-associated protein TAC-1/TACC, and require the EFA-6 MT elimination domain (MTED). Our results reveal functionally important crosstalk between cellular tubulins and cortical MT regulators in vivo.

Indexed as

Caenorhabditis elegansCaenorhabditis elegans ProteinsGuanine Nucleotide Exchange FactorsMicrotubulesTubulinAnimalsIntrinsically Disordered ProteinsMutationProtein BindingCaenorhabditis elegans ProteinsEFA-6 protein, C elegansGuanine Nucleotide Exchange FactorsIntrinsically Disordered ProteinsTubulinbiomolecular condensatescortical cytoskeletonCP: Cell biologyepidermismicrotubuleTACCtubulin heterodimer

Identifiers

PMID39305484
PMCPMC11972086

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.