ArticleNature methods2024
A modular chemigenetic calcium indicator for multiplexed in vivo functional imaging.
Article in Nature methods, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed.
- A chemigenetic endogenous Ras activity biosensor for live-cell pharmacology.Nature chemical biology · 2026Article
- CLOK: a chemigenetic multicolor labeling system to visualize neuronal birthdate and circuit integration.Nature neuroscience · 2026Article
- PrismaLuc luciferase enables tunable near-infrared bioluminescence forRSC chemical biology · 2026Article
- Genetically encoded tools for tracking metabolites in live cells.Biochemical Society transactions · 2026Review
- Crosstalk-free optical indicator-actuator combinations for bidirectional optogenetics.npj biomedical innovations · 2026Review
- Tension TRAAKer: a chemigenetic fluorescent membrane tension reporter.bioRxiv : the preprint server for biology · 2026Article
- Precise calcium-to-spike inference using biophysical generative models.bioRxiv : the preprint server for biology · 2026Article
- Illuminating compartmentalized AMPK signaling in single cells.The Biochemical journal · 2026Review
- A membrane-permeable small molecule biosensor accesses intractable cells and animals without genetic manipulation.bioRxiv : the preprint server for biology · 2026Article
- A high-affinity split-HaloTag for live-cell protein labeling.Nature communications · 2026Article
- Chemical fluorophores for fluorescence lifetime imaging.Chemical Society reviews · 2026Review
- Multi-scale imaging and recording of in-vivo neural activity.BMB reports · 2026Review
- Steric confinement-induced emission probe for monitoring protein conformations in live cells.Communications chemistry · 2026Article
- Fast, Bright, and Reversible Fluorescent Labeling of Rhodamine-Binding Proteins.Journal of the American Chemical Society · 2026Article
- Protein engineering: status report.Protein engineering, design & selection : PEDS · 2026Review
- Imaging mitochondrial membrane potential via concentration-dependent fluorescence lifetime changes.Nature communications · 2025Article
- Synthetic fluorophores for live-cell fluorescence microscopy and biosensing.Nature chemical biology · 2025Review
- Article
- Calcium Dynamics in Astrocyte-Neuron Communication from Intracellular to Extracellular Signaling.Cells · 2025Review
- Hyperspectral Fluorescence Imaging with a New Polarity-Ultrasensitive Fluorescent Probe.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
Corrections and comments
- Update of
Authors and funding
21 authors.
Funding
Abstract
Genetically encoded fluorescent calcium indicators allow cellular-resolution recording of physiology. However, bright, genetically targetable indicators that can be multiplexed with existing tools in vivo are needed for simultaneous imaging of multiple signals. Here we describe WHaloCaMP, a modular chemigenetic calcium indicator built from bright dye-ligands and protein sensor domains. Fluorescence change in WHaloCaMP results from reversible quenching of the bound dye via a strategically placed tryptophan. WHaloCaMP is compatible with rhodamine dye-ligands that fluoresce from green to near-infrared, including several that efficiently label the brain in animals. When bound to a near-infrared dye-ligand, WHaloCaMP shows a 7× increase in fluorescence intensity and a 2.1-ns increase in fluorescence lifetime upon calcium binding. We use WHaloCaMP1a to image Ca
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.