Architecture of CTPS filament networks revealed by cryo-electron tomography.

You Fu, Chen-Jun Guo, Zhi-Jie Liu, Ji-Long Liu

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Article in Experimental cell research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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4 authors.

You FuSchool of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China; iHuman Institute, ShanghaiTech University, Shanghai, 201210, China.

Chen-Jun GuoSchool of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China.

Zhi-Jie LiuSchool of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China; iHuman Institute, ShanghaiTech University, Shanghai, 201210, China.

Ji-Long LiuSchool of Life Science and Technology, ShanghaiTech University, Shanghai, 201210, China; Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, OX1 3PT, UK. Electronic address: liujl3@shanghaitech.edu.cn.

Funding

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6 · The paper itself

Abstract

The cytoophidium is a novel type of membraneless organelle, first observed in the ovaries of Drosophila using fluorescence microscopy. In vitro, purified Drosophila melanogaster CTPS (dmCTPS) can form metabolic filaments under the presence of either substrates or products, and their structures that have been analyzed using cryo-electron microscopy (cryo-EM). These dmCTPS filaments are considered the fundamental units of cytoophidia. However, due to the resolution gap between light and electron microscopy, the precise assembly pattern of cytoophidia remains unclear. In this study, we find that dmCTPS filaments can spontaneously assemble in vitro, forming network structures that reach micron-scale dimensions. Using cryo-electron tomography (cryo-ET), we reconstruct the network structures formed by dmCTPS filaments under substrate or product binding conditions and elucidate their assembly process. The dmCTPS filaments initially form structural bundles, which then further assemble into larger networks. By identifying, tracking, and statistically analyzing the filaments, we observed distinct characteristics of the structural bundles formed under different conditions. This study provides the first systematic analysis of dmCTPS filament networks, offering new insights into the relationship between cytoophidia and metabolic filaments.

Indexed as

Cryoelectron MicroscopyDrosophila melanogasterElectron Microscope TomographyAnimalsCarbon-Nitrogen LigasesCytoskeletonDrosophila ProteinsFemaleOrganellesCarbon-Nitrogen LigasesCTP synthetaseDrosophila ProteinsCryo-electron microscopyCryo-electron tomographyCTPSCTPS filament networkCytoophidiumMetabolic filament

Identifiers

PMID39303837

DOI10.1016/j.yexcr.2024.114262

PMCPMC7618999

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