Evidence map›Paper›PMID 39303801›Full record

ArticleRespiratory physiology & neurobiology2025

Endomorphin-2 (Endo2) and substance P (SubP) co-application attenuates SubP-induced excitation and alters frequency plasticity in neonatal rat in vitro preparations.

Stephen M Johnson, Sarah M Johnson, Jyoti J Watters, Tracy L Baker

Abstract read
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Article in Respiratory physiology & neurobiology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Stephen M JohnsonDepartment of Comparative Biosciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, United States. Electronic address: stephen.m.johnson@wisc.edu.
Sarah M JohnsonDepartment of Comparative Biosciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, United States.
Jyoti J WattersDepartment of Comparative Biosciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, United States.
Tracy L BakerDepartment of Comparative Biosciences, School of Veterinary Medicine, University of Wisconsin-Madison, Madison, WI, United States.

Funding

The relationship between sleep apnea and Alzheimer's disease in a unique mouse model: role for microgliaR01HL142752 · NHLBI · UNIVERSITY OF WISCONSIN-MADISON · PI BAKER, TRACY L, WATTERS, JYOTI J · 2019 to 2022
$3.0M
Regulation of microglial plasticity by TLR4 and microRNAsR01NS085226 · NINDS · UNIVERSITY OF WISCONSIN-MADISON · PI WATTERS, JYOTI J · 2015 to 2019
$1.7M
NHLBI NIH HHS R01 HL142752NINDS NIH HHS R01 NS085226
6 · The paper itself

Abstract

Substance P (SubP) and endomorphin-2 (Endo2) are co-localized presynaptically in vesicles of neurons adjacent to inspiratory rhythm-generating pre-Botzinger Complex (preBotC) neurons but the effects of co-released SubP and Endo2 on respiratory motor control are not known. To address this question, SubP alone or a combination of SubP and Endo2 (SubP/Endo2) were bath-applied in a sustained (15-min) or intermittent (5-min application, 5-min washout, x3) pattern at 10-100 nM to neonatal rat brainstem-spinal cord preparations. During neuropeptide application, SubP/Endo2 co-applications generally attenuated SubP-induced increases in burst frequency and decreases in burst amplitude. With respect to frequency plasticity (long-lasting increase in burst frequency 60 min post-neuropeptide application), SubP-induced frequency plasticity was increased with sustained SubP/Endo2 co-applications at 20 and 100 nM. Intermittent SubP/Endo2 co-applications tended to decrease the level of frequency plasticity induced by intermittent SubP alone applications. SubP/Endo2 co-applications revealed potentially new functions for neurokinin-1 (NK1R) and mu-opioid (MOR) receptors on respiratory rhythm-generating medullary neurons.

Indexed as

Animals, NewbornBrain StemNeuronal PlasticityOligopeptidesRats, Sprague-DawleySpinal CordSubstance PAction PotentialsAnimalsDose-Response Relationship, DrugIn Vitro TechniquesNeuronsRatsendomorphin 2OligopeptidesSubstance PMu-opioid receptorNeonatal ratNeurokinin-1 receptorNeuromodulationNeuroplasticityRespiratory motor control

Identifiers

PMID39303801
PMCPMC11614698

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.