Evidence map›Paper›PMID 39296406›Full record

ArticleBiomedical optics express2024

Mechanically sheared axially swept light-sheet microscopy.

Jinlong Lin, Dushyant Mehra, Zach Marin, Xiaoding Wang, Hazel M Borges, Qionghua Shen, Seweryn Gałecki, John Haug, Derek H Abbott, Kevin M Dean

Abstract read
In one paragraph

Article in Biomedical optics express, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors.

Jinlong LinLyda Hill Department of Bioinformatics, UT Southwestern Medical Center, 6000 Harry Hines Blvd, Dallas, TX 75390, USA.
Dushyant MehraLyda Hill Department of Bioinformatics, UT Southwestern Medical Center, 6000 Harry Hines Blvd, Dallas, TX 75390, USA.
Zach MarinLyda Hill Department of Bioinformatics, UT Southwestern Medical Center, 6000 Harry Hines Blvd, Dallas, TX 75390, USA.
Xiaoding WangLyda Hill Department of Bioinformatics, UT Southwestern Medical Center, 6000 Harry Hines Blvd, Dallas, TX 75390, USA.
Hazel M BorgesLyda Hill Department of Bioinformatics, UT Southwestern Medical Center, 6000 Harry Hines Blvd, Dallas, TX 75390, USA.
Qionghua ShenLyda Hill Department of Bioinformatics, UT Southwestern Medical Center, 6000 Harry Hines Blvd, Dallas, TX 75390, USA.
Seweryn GałeckiLyda Hill Department of Bioinformatics, UT Southwestern Medical Center, 6000 Harry Hines Blvd, Dallas, TX 75390, USA.
John HaugLyda Hill Department of Bioinformatics, UT Southwestern Medical Center, 6000 Harry Hines Blvd, Dallas, TX 75390, USA.
Derek H AbbottLyda Hill Department of Bioinformatics, UT Southwestern Medical Center, 6000 Harry Hines Blvd, Dallas, TX 75390, USA.
Kevin M DeanLyda Hill Department of Bioinformatics, UT Southwestern Medical Center, 6000 Harry Hines Blvd, Dallas, TX 75390, USA.ORCID https://orcid.org/0000-0003-0839-2320

Funding

Technical Development Unit 2: Intelligent Hyperspectral Imaging of Subcellular Molecular States at the Whole Organ LevelU54CA268072 · NCI · UT SOUTHWESTERN MEDICAL CENTER · PI SEAN J MORRISON · 2021 to 2026
$9.1M
UTSW-UNC Center for Cell Signaling AnalysisRM1GM145399 · NIGMS · UT SOUTHWESTERN MEDICAL CENTER · PI Kevin Michael Dean, Klaus M. Hahn · 2022 to 2026
$6.2M
NCI NIH HHS U54 CA268072NIGMS NIH HHS RM1 GM145399
6 · The paper itself

Abstract

We present a mechanically sheared image acquisition format for upright and open-top light-sheet microscopes that automatically places data in its proper spatial context. This approach, which reduces computational post-processing and eliminates unnecessary interpolation or duplication of the data, is demonstrated on an upright variant of axially swept light-sheet microscopy (ASLM) that achieves a field of view, measuring 774 × 435 microns, that is 3.2-fold larger than previous models and a raw and isotropic resolution of ∼460 nm. Combined, we demonstrate the power of this approach by imaging sub-diffraction beads, cleared biological tissues, and expanded specimens.

Identifiers

PMID39296406
PMCPMC11407235

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.